Protein domain-dependent vesiculation of Lipoprotein A, a protein that is important in cell wall synthesis and fitness of the human respiratory pathogen Haemophilus influenzae
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Protein domain-dependent vesiculation of Lipoprotein A, a protein that is important in cell wall synthesis and fitness of the human respiratory pathogen Haemophilus influenzae. / Jalalvand, Farshid; Su, Yu-Ching; Manat, Guillaume; Chernobrovkin, Alexey; Kadari, Mahendar; Jonsson, Sandra; Janousková, Martina; Rutishauser, Dorothea; Semsey, Szabolcs; Løbner-Olesen, Anders; Sandblad, Linda; Flärdh, Klas; Mengin-Lecreulx, Dominique; Zubarev, Roman A.; Riesbeck, Kristian.
In: Frontiers in Cellular and Infection Microbiology, Vol. 12, 984955, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Protein domain-dependent vesiculation of Lipoprotein A, a protein that is important in cell wall synthesis and fitness of the human respiratory pathogen Haemophilus influenzae
AU - Jalalvand, Farshid
AU - Su, Yu-Ching
AU - Manat, Guillaume
AU - Chernobrovkin, Alexey
AU - Kadari, Mahendar
AU - Jonsson, Sandra
AU - Janousková, Martina
AU - Rutishauser, Dorothea
AU - Semsey, Szabolcs
AU - Løbner-Olesen, Anders
AU - Sandblad, Linda
AU - Flärdh, Klas
AU - Mengin-Lecreulx, Dominique
AU - Zubarev, Roman A.
AU - Riesbeck, Kristian
N1 - Copyright © 2022 Jalalvand, Su, Manat, Chernobrovkin, Kadari, Jonsson, Janousková, Rutishauser, Semsey, Løbner-Olesen, Sandblad, Flärdh, Mengin-Lecreulx, Zubarev and Riesbeck.
PY - 2022
Y1 - 2022
N2 - The human pathogen Haemophilus influenzae causes respiratory tract infections and is commonly associated with prolonged carriage in patients with chronic obstructive pulmonary disease. Production of outer membrane vesicles (OMVs) is a ubiquitous phenomenon observed in Gram-negative bacteria including H. influenzae. OMVs play an important role in various interactions with the human host; from neutralization of antibodies and complement activation to spread of antimicrobial resistance. Upon vesiculation certain proteins are found in OMVs and some proteins are retained at the cell membrane. The mechanism for this phenomenon is not fully elucidated. We employed mass spectrometry to study vesiculation and the fate of proteins in the outer membrane. Functional groups of proteins were differentially distributed on the cell surface and in OMVs. Despite its supposedly periplasmic and outer membrane location, we found that the peptidoglycan synthase-activator Lipoprotein A (LpoA) was accumulated in OMVs relative to membrane fractions. A mutant devoid of LpoA lost its fitness as revealed by growth and electron microscopy. Furthermore, high-pressure liquid chromatography disclosed a lower concentration (55%) of peptidoglycan in the LpoA-deficient H. influenzae compared to the parent wild type bacterium. Using an LpoA-mNeonGreen fusion protein and fluorescence microscopy, we observed that LpoA was enriched in "foci" in the cell envelope, and further located in the septum during cell division. To define the fate of LpoA, C-terminally truncated LpoA-variants were constructed, and we found that the LpoA C-terminal domain promoted optimal transportation to the OMVs as revealed by flow cytometry. Taken together, our study highlights the importance of LpoA for H. influenzae peptidoglycan biogenesis and provides novel insights into cell wall integrity and OMV production.
AB - The human pathogen Haemophilus influenzae causes respiratory tract infections and is commonly associated with prolonged carriage in patients with chronic obstructive pulmonary disease. Production of outer membrane vesicles (OMVs) is a ubiquitous phenomenon observed in Gram-negative bacteria including H. influenzae. OMVs play an important role in various interactions with the human host; from neutralization of antibodies and complement activation to spread of antimicrobial resistance. Upon vesiculation certain proteins are found in OMVs and some proteins are retained at the cell membrane. The mechanism for this phenomenon is not fully elucidated. We employed mass spectrometry to study vesiculation and the fate of proteins in the outer membrane. Functional groups of proteins were differentially distributed on the cell surface and in OMVs. Despite its supposedly periplasmic and outer membrane location, we found that the peptidoglycan synthase-activator Lipoprotein A (LpoA) was accumulated in OMVs relative to membrane fractions. A mutant devoid of LpoA lost its fitness as revealed by growth and electron microscopy. Furthermore, high-pressure liquid chromatography disclosed a lower concentration (55%) of peptidoglycan in the LpoA-deficient H. influenzae compared to the parent wild type bacterium. Using an LpoA-mNeonGreen fusion protein and fluorescence microscopy, we observed that LpoA was enriched in "foci" in the cell envelope, and further located in the septum during cell division. To define the fate of LpoA, C-terminally truncated LpoA-variants were constructed, and we found that the LpoA C-terminal domain promoted optimal transportation to the OMVs as revealed by flow cytometry. Taken together, our study highlights the importance of LpoA for H. influenzae peptidoglycan biogenesis and provides novel insights into cell wall integrity and OMV production.
KW - Humans
KW - Haemophilus influenzae/metabolism
KW - Protein Domains
KW - Bacterial Outer Membrane Proteins/metabolism
KW - Lipoprotein(a)/metabolism
KW - Peptidoglycan/metabolism
KW - Cell Wall/metabolism
KW - Anti-Infective Agents/metabolism
U2 - 10.3389/fcimb.2022.984955
DO - 10.3389/fcimb.2022.984955
M3 - Journal article
C2 - 36275016
VL - 12
JO - Frontiers in Cellular and Infection Microbiology
JF - Frontiers in Cellular and Infection Microbiology
SN - 2235-2988
M1 - 984955
ER -
ID: 323547982