Pyruvate kinase M2 represses thermogenic gene expression in brown adipocytes

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Pyruvate kinase M2 represses thermogenic gene expression in brown adipocytes. / Isidor, Marie S.; Winther, Sally; Markussen, Lasse K.; Basse, Astrid L.; Quistorff, Bjørn; Nedergaard, Jan; Emanuelli, Brice; Hansen, Jacob B.

In: FEBS Letters, Vol. 594, No. 7, 2020, p. 1218-1225.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Isidor, MS, Winther, S, Markussen, LK, Basse, AL, Quistorff, B, Nedergaard, J, Emanuelli, B & Hansen, JB 2020, 'Pyruvate kinase M2 represses thermogenic gene expression in brown adipocytes', FEBS Letters, vol. 594, no. 7, pp. 1218-1225. https://doi.org/10.1002/1873-3468.13716

APA

Isidor, M. S., Winther, S., Markussen, L. K., Basse, A. L., Quistorff, B., Nedergaard, J., Emanuelli, B., & Hansen, J. B. (2020). Pyruvate kinase M2 represses thermogenic gene expression in brown adipocytes. FEBS Letters, 594(7), 1218-1225. https://doi.org/10.1002/1873-3468.13716

Vancouver

Isidor MS, Winther S, Markussen LK, Basse AL, Quistorff B, Nedergaard J et al. Pyruvate kinase M2 represses thermogenic gene expression in brown adipocytes. FEBS Letters. 2020;594(7):1218-1225. https://doi.org/10.1002/1873-3468.13716

Author

Isidor, Marie S. ; Winther, Sally ; Markussen, Lasse K. ; Basse, Astrid L. ; Quistorff, Bjørn ; Nedergaard, Jan ; Emanuelli, Brice ; Hansen, Jacob B. / Pyruvate kinase M2 represses thermogenic gene expression in brown adipocytes. In: FEBS Letters. 2020 ; Vol. 594, No. 7. pp. 1218-1225.

Bibtex

@article{93a7166ac3dc41e9a2cb58b3ed8a7ee2,
title = "Pyruvate kinase M2 represses thermogenic gene expression in brown adipocytes",
abstract = "Utilizing the thermogenic capacity of brown adipose tissue is a potential anti-obesity strategy; therefore, the mechanisms controlling expression of thermogenesis-related genes are of interest. Pyruvate kinase (PK) catalyzes the last step of glycolysis and exists as four isoenzymes: PK, liver, PK, red blood cell, PK, muscle (PKM1 and PKM2). PKM2 has both glycolytic and nuclear functions. Here, we report that PKM2 is enriched in brown adipose compared with white adipose tissue. Specific knockdown of PKM2 in mature brown adipocytes demonstrates that silencing of PKM2 does not lead to a decrease in PK activity, but causes a robust upregulation of thermogenic uncoupling protein 1 (Ucp1) and fibroblast growth factor 21 (Fgf21) gene expression. This increase is not mediated by any of the known mechanisms for PKM2-regulated gene expression, thus implying the existence of a novel mechanism for PKM2-dependent effects on gene expression.",
keywords = "brown adipose tissue, Fgf21, glycolysis, metabolism, obesity, pyruvate kinase, thermogenesis, Ucp1",
author = "Isidor, {Marie S.} and Sally Winther and Markussen, {Lasse K.} and Basse, {Astrid L.} and Bj{\o}rn Quistorff and Jan Nedergaard and Brice Emanuelli and Hansen, {Jacob B.}",
year = "2020",
doi = "10.1002/1873-3468.13716",
language = "English",
volume = "594",
pages = "1218--1225",
journal = "F E B S Letters",
issn = "0014-5793",
publisher = "JohnWiley & Sons Ltd",
number = "7",

}

RIS

TY - JOUR

T1 - Pyruvate kinase M2 represses thermogenic gene expression in brown adipocytes

AU - Isidor, Marie S.

AU - Winther, Sally

AU - Markussen, Lasse K.

AU - Basse, Astrid L.

AU - Quistorff, Bjørn

AU - Nedergaard, Jan

AU - Emanuelli, Brice

AU - Hansen, Jacob B.

PY - 2020

Y1 - 2020

N2 - Utilizing the thermogenic capacity of brown adipose tissue is a potential anti-obesity strategy; therefore, the mechanisms controlling expression of thermogenesis-related genes are of interest. Pyruvate kinase (PK) catalyzes the last step of glycolysis and exists as four isoenzymes: PK, liver, PK, red blood cell, PK, muscle (PKM1 and PKM2). PKM2 has both glycolytic and nuclear functions. Here, we report that PKM2 is enriched in brown adipose compared with white adipose tissue. Specific knockdown of PKM2 in mature brown adipocytes demonstrates that silencing of PKM2 does not lead to a decrease in PK activity, but causes a robust upregulation of thermogenic uncoupling protein 1 (Ucp1) and fibroblast growth factor 21 (Fgf21) gene expression. This increase is not mediated by any of the known mechanisms for PKM2-regulated gene expression, thus implying the existence of a novel mechanism for PKM2-dependent effects on gene expression.

AB - Utilizing the thermogenic capacity of brown adipose tissue is a potential anti-obesity strategy; therefore, the mechanisms controlling expression of thermogenesis-related genes are of interest. Pyruvate kinase (PK) catalyzes the last step of glycolysis and exists as four isoenzymes: PK, liver, PK, red blood cell, PK, muscle (PKM1 and PKM2). PKM2 has both glycolytic and nuclear functions. Here, we report that PKM2 is enriched in brown adipose compared with white adipose tissue. Specific knockdown of PKM2 in mature brown adipocytes demonstrates that silencing of PKM2 does not lead to a decrease in PK activity, but causes a robust upregulation of thermogenic uncoupling protein 1 (Ucp1) and fibroblast growth factor 21 (Fgf21) gene expression. This increase is not mediated by any of the known mechanisms for PKM2-regulated gene expression, thus implying the existence of a novel mechanism for PKM2-dependent effects on gene expression.

KW - brown adipose tissue

KW - Fgf21

KW - glycolysis

KW - metabolism

KW - obesity

KW - pyruvate kinase

KW - thermogenesis

KW - Ucp1

U2 - 10.1002/1873-3468.13716

DO - 10.1002/1873-3468.13716

M3 - Journal article

C2 - 31823361

AN - SCOPUS:85077076425

VL - 594

SP - 1218

EP - 1225

JO - F E B S Letters

JF - F E B S Letters

SN - 0014-5793

IS - 7

ER -

ID: 239203931