Quantifying Microbial Diversity: Morphotypes, 16S rRNA Genes, and Carotenoids of Oxygenic Phototrophs in Microbial Mats

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Standard

Quantifying Microbial Diversity : Morphotypes, 16S rRNA Genes, and Carotenoids of Oxygenic Phototrophs in Microbial Mats. / Nübel, Ulrich; Garcia-Pichel, Ferran; Kühl, Michael; Muyzer, Gerard.

In: Applied and Environmental Microbiology, Vol. 65, No. 2, 1999, p. 422-430.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Nübel, U, Garcia-Pichel, F, Kühl, M & Muyzer, G 1999, 'Quantifying Microbial Diversity: Morphotypes, 16S rRNA Genes, and Carotenoids of Oxygenic Phototrophs in Microbial Mats', Applied and Environmental Microbiology, vol. 65, no. 2, pp. 422-430. https://doi.org/10.1128/AEM.65.2.422-430.1999

APA

Nübel, U., Garcia-Pichel, F., Kühl, M., & Muyzer, G. (1999). Quantifying Microbial Diversity: Morphotypes, 16S rRNA Genes, and Carotenoids of Oxygenic Phototrophs in Microbial Mats. Applied and Environmental Microbiology, 65(2), 422-430. https://doi.org/10.1128/AEM.65.2.422-430.1999

Vancouver

Nübel U, Garcia-Pichel F, Kühl M, Muyzer G. Quantifying Microbial Diversity: Morphotypes, 16S rRNA Genes, and Carotenoids of Oxygenic Phototrophs in Microbial Mats. Applied and Environmental Microbiology. 1999;65(2):422-430. https://doi.org/10.1128/AEM.65.2.422-430.1999

Author

Nübel, Ulrich ; Garcia-Pichel, Ferran ; Kühl, Michael ; Muyzer, Gerard. / Quantifying Microbial Diversity : Morphotypes, 16S rRNA Genes, and Carotenoids of Oxygenic Phototrophs in Microbial Mats. In: Applied and Environmental Microbiology. 1999 ; Vol. 65, No. 2. pp. 422-430.

Bibtex

@article{9a357163f2844aaea834569e85a00af0,
title = "Quantifying Microbial Diversity: Morphotypes, 16S rRNA Genes, and Carotenoids of Oxygenic Phototrophs in Microbial Mats",
abstract = "We quantified the diversity of oxygenic phototrophic microorganisms present in eight hypersaline microbial mats on the basis of three cultivation-independent approaches. Morphological diversity was studied by microscopy. The diversity of carotenoids was examined by extraction from mat samples and high-pressure liquid chromatography analysis. The diversity of 16S rRNA genes from oxygenic phototrophic microorganisms was investigated by extraction of total DNA from mat samples, amplification of 16S rRNA gene segments from cyanobacteria and plastids of eukaryotic algae by phylum- specific PCR, and sequence-dependent separation of amplification products by denaturing-gradient gel electrophoresis. A numerical approach was introduced to correct for crowding the results of chromatographic and electrophoretic analyses. Diversity estimates typically varied up to twofold among mats. The congruence of richness estimates and Shannon-Weaver indices based on numbers and proportional abundances of unique morphotypes, 16S rRNA genes, and carotenoids unveiled the underlying diversity of oxygenic phototrophic microorganisms in the eight mat communities studied.",
author = "Ulrich N{\"u}bel and Ferran Garcia-Pichel and Michael K{\"u}hl and Gerard Muyzer",
year = "1999",
doi = "10.1128/AEM.65.2.422-430.1999",
language = "English",
volume = "65",
pages = "422--430",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "2",

}

RIS

TY - JOUR

T1 - Quantifying Microbial Diversity

T2 - Morphotypes, 16S rRNA Genes, and Carotenoids of Oxygenic Phototrophs in Microbial Mats

AU - Nübel, Ulrich

AU - Garcia-Pichel, Ferran

AU - Kühl, Michael

AU - Muyzer, Gerard

PY - 1999

Y1 - 1999

N2 - We quantified the diversity of oxygenic phototrophic microorganisms present in eight hypersaline microbial mats on the basis of three cultivation-independent approaches. Morphological diversity was studied by microscopy. The diversity of carotenoids was examined by extraction from mat samples and high-pressure liquid chromatography analysis. The diversity of 16S rRNA genes from oxygenic phototrophic microorganisms was investigated by extraction of total DNA from mat samples, amplification of 16S rRNA gene segments from cyanobacteria and plastids of eukaryotic algae by phylum- specific PCR, and sequence-dependent separation of amplification products by denaturing-gradient gel electrophoresis. A numerical approach was introduced to correct for crowding the results of chromatographic and electrophoretic analyses. Diversity estimates typically varied up to twofold among mats. The congruence of richness estimates and Shannon-Weaver indices based on numbers and proportional abundances of unique morphotypes, 16S rRNA genes, and carotenoids unveiled the underlying diversity of oxygenic phototrophic microorganisms in the eight mat communities studied.

AB - We quantified the diversity of oxygenic phototrophic microorganisms present in eight hypersaline microbial mats on the basis of three cultivation-independent approaches. Morphological diversity was studied by microscopy. The diversity of carotenoids was examined by extraction from mat samples and high-pressure liquid chromatography analysis. The diversity of 16S rRNA genes from oxygenic phototrophic microorganisms was investigated by extraction of total DNA from mat samples, amplification of 16S rRNA gene segments from cyanobacteria and plastids of eukaryotic algae by phylum- specific PCR, and sequence-dependent separation of amplification products by denaturing-gradient gel electrophoresis. A numerical approach was introduced to correct for crowding the results of chromatographic and electrophoretic analyses. Diversity estimates typically varied up to twofold among mats. The congruence of richness estimates and Shannon-Weaver indices based on numbers and proportional abundances of unique morphotypes, 16S rRNA genes, and carotenoids unveiled the underlying diversity of oxygenic phototrophic microorganisms in the eight mat communities studied.

U2 - 10.1128/AEM.65.2.422-430.1999

DO - 10.1128/AEM.65.2.422-430.1999

M3 - Journal article

AN - SCOPUS:2542509415

VL - 65

SP - 422

EP - 430

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 2

ER -

ID: 201682277