Regulation of ribosome synthesis in Tetrahymena pyriformis. 3. Analysis by translation in vitro of RNA isolated during nutritional shift-down and nutritional shift-up
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Regulation of ribosome synthesis in Tetrahymena pyriformis. 3. Analysis by translation in vitro of RNA isolated during nutritional shift-down and nutritional shift-up. / Andreasen, P H; Dreisig, H; Kristiansen, K.
In: European Journal of Biochemistry, Vol. 140, No. 3, 1984, p. 485-92.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Regulation of ribosome synthesis in Tetrahymena pyriformis. 3. Analysis by translation in vitro of RNA isolated during nutritional shift-down and nutritional shift-up
AU - Andreasen, P H
AU - Dreisig, H
AU - Kristiansen, K
N1 - Keywords: Animals; Culture Media; Electrophoresis; Protein Biosynthesis; RNA, Ribosomal; Ribosomal Proteins; Ribosomes; Templates, Genetic; Tetrahymena pyriformis
PY - 1984
Y1 - 1984
N2 - We have measured the levels of translatable total mRNA and ribosomal protein (r-protein) mRNAs in Tetrahymena pyriformis during nutritional shifts. After 15 min of starvation total mRNA is reduced 2-fold, and after 24 h 7.5-fold, relative to exponentially growing cells. Upon refeeding total mRNA increases rapidly reaching the level of exponentially growing cells after 2.5 h. The levels of the individual r-protein mRNAs are coordinately regulated throughout a starvation-refeeding cycle. The relative levels of r-protein mRNAs remain virtually unchanged during the first hour of starvation and then decrease gradually to 30% of the relative levels in exponentially growing cells. Following refeeding the relative levels of r-protein mRNAs increase 6-fold. Taking into account the changes in whole cell RNA, we have calculated that long-time-starved Tetrahymena cells contain only 4%, whereas cells after 3 h of refeeding contain 200% of the amount of r-protein mRNA in exponentially growing cells. The amount of r-protein mRNA thus increases 50-fold during the first 3 h of refeeding. A comparison between the relative levels of r-protein mRNAs and the relative rate of r-protein synthesis in vivo indicates that Tetrahymena employs a combination of control of translation and control of the level of r-protein mRNAs to ensure a rapid reduction in the rate of r-protein synthesis during the early period of starvation. In this period translation of r-protein mRNAs is preferentially inhibited. During refeeding the increase in the rate of r-protein synthesis parallels the increase in the abundance of r-protein mRNAs.
AB - We have measured the levels of translatable total mRNA and ribosomal protein (r-protein) mRNAs in Tetrahymena pyriformis during nutritional shifts. After 15 min of starvation total mRNA is reduced 2-fold, and after 24 h 7.5-fold, relative to exponentially growing cells. Upon refeeding total mRNA increases rapidly reaching the level of exponentially growing cells after 2.5 h. The levels of the individual r-protein mRNAs are coordinately regulated throughout a starvation-refeeding cycle. The relative levels of r-protein mRNAs remain virtually unchanged during the first hour of starvation and then decrease gradually to 30% of the relative levels in exponentially growing cells. Following refeeding the relative levels of r-protein mRNAs increase 6-fold. Taking into account the changes in whole cell RNA, we have calculated that long-time-starved Tetrahymena cells contain only 4%, whereas cells after 3 h of refeeding contain 200% of the amount of r-protein mRNA in exponentially growing cells. The amount of r-protein mRNA thus increases 50-fold during the first 3 h of refeeding. A comparison between the relative levels of r-protein mRNAs and the relative rate of r-protein synthesis in vivo indicates that Tetrahymena employs a combination of control of translation and control of the level of r-protein mRNAs to ensure a rapid reduction in the rate of r-protein synthesis during the early period of starvation. In this period translation of r-protein mRNAs is preferentially inhibited. During refeeding the increase in the rate of r-protein synthesis parallels the increase in the abundance of r-protein mRNAs.
U2 - 10.1111/j.1432-1033.1984.tb08128.x
DO - 10.1111/j.1432-1033.1984.tb08128.x
M3 - Journal article
C2 - 6426955
VL - 140
SP - 485
EP - 492
JO - FEBS Journal
JF - FEBS Journal
SN - 1742-464X
IS - 3
ER -
ID: 11368475