RNA interference in Lepidoptera: an overview of successful and unsuccessful studies and implicatons for experimantal design
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RNA interference in Lepidoptera : an overview of successful and unsuccessful studies and implicatons for experimantal design. / Terenius, Ole; Papanicolaou, Alexie; Garbutt, Jennie S.; Albrechtsen, Merete Tryde; Grimmelikhuijzen, Cornelis; Hauser, Frank; Lundmark, Krister Ove Magnus.
In: Journal of Insect Physiology, Vol. 57, No. 2, 2011, p. 231-245.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - RNA interference in Lepidoptera
T2 - an overview of successful and unsuccessful studies and implicatons for experimantal design
AU - Terenius, Ole
AU - Papanicolaou, Alexie
AU - Garbutt, Jennie S.
AU - Albrechtsen, Merete Tryde
AU - Grimmelikhuijzen, Cornelis
AU - Hauser, Frank
AU - Lundmark, Krister Ove Magnus
PY - 2011
Y1 - 2011
N2 - Gene silencing through RNA interference (RNAi) has revolutionized the study of gene function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) RNAi has many times proven to be difficult to achieve. Most of the negative results have been anecdotal and the positive experiments have not been collected in such a way that they are possible to analyze. In this review, we have collected detailed data from more than 150 experiments including all to date published and many unpublished experiments. Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involved in immunity. On the contrary, gene expression in epidermal tissues seems to be most difficult to silence. In addition, gene silencing by feeding dsRNA requires high concentrations for success. Possible causes for the variability of success in RNAi experiments in Lepidoptera are discussed. The review also points to a need to further investigate the mechanism of RNAi in lepidopteran insects and its possible connection to the innate immune response. Our general understanding of RNAi in Lepidoptera will be further aided in the future as our public database at http://insectacentral.org/RNAi will continue to gather information on RNAi experiments. 2010 Elsevier Ltd. All rights reserved.
AB - Gene silencing through RNA interference (RNAi) has revolutionized the study of gene function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) RNAi has many times proven to be difficult to achieve. Most of the negative results have been anecdotal and the positive experiments have not been collected in such a way that they are possible to analyze. In this review, we have collected detailed data from more than 150 experiments including all to date published and many unpublished experiments. Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involved in immunity. On the contrary, gene expression in epidermal tissues seems to be most difficult to silence. In addition, gene silencing by feeding dsRNA requires high concentrations for success. Possible causes for the variability of success in RNAi experiments in Lepidoptera are discussed. The review also points to a need to further investigate the mechanism of RNAi in lepidopteran insects and its possible connection to the innate immune response. Our general understanding of RNAi in Lepidoptera will be further aided in the future as our public database at http://insectacentral.org/RNAi will continue to gather information on RNAi experiments. 2010 Elsevier Ltd. All rights reserved.
U2 - 10.1016/j.jinsphys.2010.11.006
DO - 10.1016/j.jinsphys.2010.11.006
M3 - Journal article
VL - 57
SP - 231
EP - 245
JO - Journal of Insect Physiology
JF - Journal of Insect Physiology
SN - 0022-1910
IS - 2
ER -
ID: 34480492