RNA-Seq for Bacterial Gene Expression
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RNA-Seq for Bacterial Gene Expression. / Poulsen, Line Dahl; Vinther, Jeppe.
In: Current Protocols in Nucleic Acid Chemistry, Vol. 73, No. 1, e55, 2018, p. 1-12.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - RNA-Seq for Bacterial Gene Expression
AU - Poulsen, Line Dahl
AU - Vinther, Jeppe
PY - 2018
Y1 - 2018
N2 - RNA sequencing (RNA-seq) has become the preferred method for global quantification of bacterial gene expression. With the continued improvements in sequencing technology and data analysis tools, the most labor-intensive and expensive part of an RNA-seq experiment is the preparation of sequencing libraries, which is also essential for the quality of the data obtained. Here, we present a straightforward and inexpensive basic protocol for preparation of strand-specific RNA-seq libraries from bacterial RNA as well as a computational pipeline for the data analysis of sequencing reads. The protocol is based on the Illumina platform and allows easy multiplexing of samples and the removal of sequencing reads that are PCR duplicates.
AB - RNA sequencing (RNA-seq) has become the preferred method for global quantification of bacterial gene expression. With the continued improvements in sequencing technology and data analysis tools, the most labor-intensive and expensive part of an RNA-seq experiment is the preparation of sequencing libraries, which is also essential for the quality of the data obtained. Here, we present a straightforward and inexpensive basic protocol for preparation of strand-specific RNA-seq libraries from bacterial RNA as well as a computational pipeline for the data analysis of sequencing reads. The protocol is based on the Illumina platform and allows easy multiplexing of samples and the removal of sequencing reads that are PCR duplicates.
KW - library construction
KW - next-generation sequencing
KW - RNA-seq
U2 - 10.1002/cpnc.55
DO - 10.1002/cpnc.55
M3 - Journal article
C2 - 29927111
AN - SCOPUS:85054136058
VL - 73
SP - 1
EP - 12
JO - Current Protocols in Nucleic Acid Chemistry
JF - Current Protocols in Nucleic Acid Chemistry
SN - 1934-9270
IS - 1
M1 - e55
ER -
ID: 210160122