Setting up Schizosaccharomyces pombe crosses/matings
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Setting up Schizosaccharomyces pombe crosses/matings. / Ekwall, Karl; Thon, Genevieve.
In: Cold Spring Harbor Protocols, Vol. 2017, No. 7, 07.2017, p. 533-536.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Setting up Schizosaccharomyces pombe crosses/matings
AU - Ekwall, Karl
AU - Thon, Genevieve
PY - 2017/7
Y1 - 2017/7
N2 - Here we provide methods for setting up standard crosses with Schizosaccharomyces pombe strains. All strain genotypes and pedigrees should be recorded in a laboratory strain book. Matings between two haploid strains of interest are induced on solid medium poor in nitrogen. Usually, sporulation agar (SPA) plates are preferred, but for difficult matings it is advisable to try several mating media in parallel because one medium might allow for more efficient mating. Protoplast fusion can be used to produce zygotes from sterile mutants that fail to mate.
AB - Here we provide methods for setting up standard crosses with Schizosaccharomyces pombe strains. All strain genotypes and pedigrees should be recorded in a laboratory strain book. Matings between two haploid strains of interest are induced on solid medium poor in nitrogen. Usually, sporulation agar (SPA) plates are preferred, but for difficult matings it is advisable to try several mating media in parallel because one medium might allow for more efficient mating. Protoplast fusion can be used to produce zygotes from sterile mutants that fail to mate.
UR - http://www.scopus.com/inward/record.url?scp=85021759545&partnerID=8YFLogxK
U2 - 10.1101/pdb.prot091694
DO - 10.1101/pdb.prot091694
M3 - Journal article
C2 - 28679701
AN - SCOPUS:85021759545
VL - 2017
SP - 533
EP - 536
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
SN - 1940-3402
IS - 7
ER -
ID: 181453491