Short-term kinetics of rRNA degradation in Escherichia coli upon starvation for carbon, amino acid, or phosphate
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Short-term kinetics of rRNA degradation in Escherichia coli upon starvation for carbon, amino acid, or phosphate. / Fessler, Mathias; Gummesson, Bertil; Charbon, Godefroid; Svenningsen, Sine Lo; Sørensen, Michael Askvad.
In: Molecular Microbiology, Vol. 113, No. 5, 2020, p. 951-963.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Short-term kinetics of rRNA degradation in Escherichia coli upon starvation for carbon, amino acid, or phosphate
AU - Fessler, Mathias
AU - Gummesson, Bertil
AU - Charbon, Godefroid
AU - Svenningsen, Sine Lo
AU - Sørensen, Michael Askvad
PY - 2020
Y1 - 2020
N2 - Ribosomes are absolutely essential for growth but are, on the other hand, energetically costly to produce. Therefore, it is important to adjust the cellular ribosome levels according to the environmental conditions in order to obtain the highest possible growth rate while avoiding energy wastage on excess ribosome biosynthesis. Here we show, by three different methods, that the ribosomal RNA content of Escherichia coli is downregulated within minutes of the removal of an essential nutrient from the growth medium, or after transcription initiation is inhibited. The kinetics of the ribosomal RNA reduction vary depending on which nutrient the cells are starved for. The number of ribosomes per OD unit of cells is roughly halved after 80 minutes of starvation for isoleucine or phosphate, whilst the ribosome reduction is less extensive when the cells are starved for glucose. Collectively, the results presented here support the simple model proposed previously, which identifies inactive ribosomal subunits as the substrates for degradation, since the most substantial rRNA degradation is observed under the starvation conditions that most directly affect protein synthesis.
AB - Ribosomes are absolutely essential for growth but are, on the other hand, energetically costly to produce. Therefore, it is important to adjust the cellular ribosome levels according to the environmental conditions in order to obtain the highest possible growth rate while avoiding energy wastage on excess ribosome biosynthesis. Here we show, by three different methods, that the ribosomal RNA content of Escherichia coli is downregulated within minutes of the removal of an essential nutrient from the growth medium, or after transcription initiation is inhibited. The kinetics of the ribosomal RNA reduction vary depending on which nutrient the cells are starved for. The number of ribosomes per OD unit of cells is roughly halved after 80 minutes of starvation for isoleucine or phosphate, whilst the ribosome reduction is less extensive when the cells are starved for glucose. Collectively, the results presented here support the simple model proposed previously, which identifies inactive ribosomal subunits as the substrates for degradation, since the most substantial rRNA degradation is observed under the starvation conditions that most directly affect protein synthesis.
U2 - 10.1111/mmi.14462
DO - 10.1111/mmi.14462
M3 - Journal article
C2 - 31960524
VL - 113
SP - 951
EP - 963
JO - Molecular Microbiology
JF - Molecular Microbiology
SN - 0950-382X
IS - 5
ER -
ID: 234455049