Similar sponge-associated bacteria can be acquired via both vertical and horizontal transmission
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Similar sponge-associated bacteria can be acquired via both vertical and horizontal transmission. / Sipkema, Detmer; de Caralt, Sònia; Morillo, Jose A; Abu Al-Soud, Waleed; Sørensen, Søren Johannes; Smidt, Hauke; Uriz, María J.
In: Environmental Microbiology, Vol. 17, No. 10, 2015, p. 3807-3821.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Similar sponge-associated bacteria can be acquired via both vertical and horizontal transmission
AU - Sipkema, Detmer
AU - de Caralt, Sònia
AU - Morillo, Jose A
AU - Abu Al-Soud, Waleed
AU - Sørensen, Søren Johannes
AU - Smidt, Hauke
AU - Uriz, María J
N1 - This article is protected by copyright. All rights reserved.
PY - 2015
Y1 - 2015
N2 - Marine sponges host diverse communities of microorganisms that are often vertically transmitted from mother to oocyte or embryo. Horizontal transmission has often been proposed to co-occur in marine sponges, but the mechanism is poorly understood. To assess the impact of the mode of transmission on the microbial assemblages of sponges, we analysed the microbiota in sympatric sponges that have previously been reported to acquire bacteria via either vertical (Corticium candelabrum and Crambe crambe) or horizontal transmission (Petrosia ficiformis). The comparative study was performed by PCR-DGGE and pyrosequencing of barcoded PCR-amplified 16S rRNA gene fragments. We found that P. ficiformis and C. candelabrum each harbor their own species-specific bacteria, but they are similar to other high-microbial-abundance sponges, while the low-microbial-abundance sponge C. crambe hosts microbiota of a very different phylogenetic signature. In addition, nearly 50% of the reads obtained from P. ficiformis were most closely related to bacteria that were previously reported to be vertically transmitted in other sponges and comprised vertical-horizontal transmission phylogenetic clusters (VHT clusters). Therefore, our results provide evidence for the hypothesis that similar sponge-associated bacteria can be acquired via both vertical and horizontal transmission.
AB - Marine sponges host diverse communities of microorganisms that are often vertically transmitted from mother to oocyte or embryo. Horizontal transmission has often been proposed to co-occur in marine sponges, but the mechanism is poorly understood. To assess the impact of the mode of transmission on the microbial assemblages of sponges, we analysed the microbiota in sympatric sponges that have previously been reported to acquire bacteria via either vertical (Corticium candelabrum and Crambe crambe) or horizontal transmission (Petrosia ficiformis). The comparative study was performed by PCR-DGGE and pyrosequencing of barcoded PCR-amplified 16S rRNA gene fragments. We found that P. ficiformis and C. candelabrum each harbor their own species-specific bacteria, but they are similar to other high-microbial-abundance sponges, while the low-microbial-abundance sponge C. crambe hosts microbiota of a very different phylogenetic signature. In addition, nearly 50% of the reads obtained from P. ficiformis were most closely related to bacteria that were previously reported to be vertically transmitted in other sponges and comprised vertical-horizontal transmission phylogenetic clusters (VHT clusters). Therefore, our results provide evidence for the hypothesis that similar sponge-associated bacteria can be acquired via both vertical and horizontal transmission.
U2 - 10.1111/1462-2920.12827
DO - 10.1111/1462-2920.12827
M3 - Journal article
C2 - 25732544
VL - 17
SP - 3807
EP - 3821
JO - Environmental Microbiology
JF - Environmental Microbiology
SN - 1462-2912
IS - 10
ER -
ID: 135220491