Single-cell sequencing analysis characterizes common and cell-lineage-specific mutations in a muscle-invasive bladder cancer
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Single-cell sequencing analysis characterizes common and cell-lineage-specific mutations in a muscle-invasive bladder cancer. / Li, Yingrui; Xu, Xun; Song, Luting; Hou, Yong; Li, Zesong; Tsang, Shirley; Li, Fuqiang; Im, Kate; Wu, Kui; Wu, Hanjie; Ye, Xiaofei; Li, Guibo; Wang, Linlin; Zhang, Bo; Liang, Jie; Xie, Wei; Wu, Renhua; Jiang, Hui; Liu, Xiao; Yu, Chang; Zheng, Hancheng; Jian, Min; Nie, Liping; Wan, Lei; Shi, Min; Sun, Xiaojuan; Tang, Aifa; Guo, Guangwu; Gui, Yaoting; Cai, Zhiming; Li, Jingxiang; Wang, Wen; Lu, Zuhong; Zhang, Xiuqing; Bolund, Lars; Kristiansen, Karsten; Wang, Jian; Yang, Huanming; Dean, Michael; Wang, Jun.
In: GigaScience, Vol. 1, No. 12, 2012.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Single-cell sequencing analysis characterizes common and cell-lineage-specific mutations in a muscle-invasive bladder cancer
AU - Li, Yingrui
AU - Xu, Xun
AU - Song, Luting
AU - Hou, Yong
AU - Li, Zesong
AU - Tsang, Shirley
AU - Li, Fuqiang
AU - Im, Kate
AU - Wu, Kui
AU - Wu, Hanjie
AU - Ye, Xiaofei
AU - Li, Guibo
AU - Wang, Linlin
AU - Zhang, Bo
AU - Liang, Jie
AU - Xie, Wei
AU - Wu, Renhua
AU - Jiang, Hui
AU - Liu, Xiao
AU - Yu, Chang
AU - Zheng, Hancheng
AU - Jian, Min
AU - Nie, Liping
AU - Wan, Lei
AU - Shi, Min
AU - Sun, Xiaojuan
AU - Tang, Aifa
AU - Guo, Guangwu
AU - Gui, Yaoting
AU - Cai, Zhiming
AU - Li, Jingxiang
AU - Wang, Wen
AU - Lu, Zuhong
AU - Zhang, Xiuqing
AU - Bolund, Lars
AU - Kristiansen, Karsten
AU - Wang, Jian
AU - Yang, Huanming
AU - Dean, Michael
AU - Wang, Jun
PY - 2012
Y1 - 2012
N2 - BACKGROUND:Cancers arise through an evolutionary process in which cell populations are subjected to selection; however, to date, the process of bladder cancer, which is one of the most common cancers in the world, remains unknown at a single-cell level.RESULTS:We carried out single-cell exome sequencing of 66 individual tumor cells from a muscle-invasive bladder transitional cell carcinoma (TCC). Analyses of the somatic mutant allele frequency spectrum and clonal structure revealed that the tumor cells were derived from a single ancestral cell, but that subsequent evolution occurred, leading to two distinct tumor cell subpopulations. By analyzing recurrently mutant genes in an additional cohort of 99 TCC tumors, we identified genes that might play roles in the maintenance of the ancestral clone and in the muscle-invasive capability of subclones of this bladder cancer, respectively.CONCLUSIONS:This work provides a new approach of investigating the genetic details of bladder tumoral changes at the single-cell level and a new method for assessing bladder cancer evolution at a cell-population level.
AB - BACKGROUND:Cancers arise through an evolutionary process in which cell populations are subjected to selection; however, to date, the process of bladder cancer, which is one of the most common cancers in the world, remains unknown at a single-cell level.RESULTS:We carried out single-cell exome sequencing of 66 individual tumor cells from a muscle-invasive bladder transitional cell carcinoma (TCC). Analyses of the somatic mutant allele frequency spectrum and clonal structure revealed that the tumor cells were derived from a single ancestral cell, but that subsequent evolution occurred, leading to two distinct tumor cell subpopulations. By analyzing recurrently mutant genes in an additional cohort of 99 TCC tumors, we identified genes that might play roles in the maintenance of the ancestral clone and in the muscle-invasive capability of subclones of this bladder cancer, respectively.CONCLUSIONS:This work provides a new approach of investigating the genetic details of bladder tumoral changes at the single-cell level and a new method for assessing bladder cancer evolution at a cell-population level.
U2 - 10.1186/2047-217X-1-12
DO - 10.1186/2047-217X-1-12
M3 - Journal article
C2 - 23587365
VL - 1
JO - GigaScience
JF - GigaScience
SN - 2047-217X
IS - 12
ER -
ID: 40906148