Small RNA target genes and regulatory connections in the Vibrio cholerae quorum sensing system

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

The two-component quorum sensing (QS) system, first described in the marine bacterium Vibrio harveyi and evolutionarily conserved among members of the genus Vibrio, has been best studied in the human pathogen Vibrio cholerae (1, 2). In the V. cholerae QS system, the response to the accumulation of extracellular autoinducers triggers a signaling cascade resulting in the transcription of four small regulatory RNAs (sRNAs). Our results support the model that the QS sRNAs bind to the 5' untranslated region of multiple mRNAs and alter the fate of one in a positive manner and several others in a negative manner. This mechanism ensures the proper timing of the QS response, which includes the expression of traits critical for virulence and for the formation of biofilms (2-6).
Original languageEnglish
Title of host publicationQuorum sensing : Methods and protocols
EditorsKendra P. Rumbaugh
Number of pages18
Volume692
PublisherHumana Press
Publication date2011
Pages189-206
Chapter14
ISBN (Print)978-1-60761-970-3
ISBN (Electronic)978-1-60761-971-0
DOIs
Publication statusPublished - 2011
SeriesMethods in Molecular Biology
ISSN1064-3745

    Research areas

  • Base Sequence, Binding Sites, Blotting, Northern, Computational Biology, Genes, Bacterial, Green Fluorescent Proteins, Kinetics, Mutagenesis, Site-Directed, Protein Biosynthesis, Quorum Sensing, RNA Splicing, RNA, Messenger, RNA, Untranslated, Reproducibility of Results, Vibrio cholerae

ID: 43239210