Spore analysis and tetrad dissection of Schizosaccharomyces pombe

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Spore analysis and tetrad dissection of Schizosaccharomyces pombe. / Ekwall, Karl; Thon, Genevieve.

In: Cold Spring Harbor Protocols, Vol. 2017, No. 7, 07.2017, p. 541-544.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ekwall, K & Thon, G 2017, 'Spore analysis and tetrad dissection of Schizosaccharomyces pombe', Cold Spring Harbor Protocols, vol. 2017, no. 7, pp. 541-544. https://doi.org/10.1101/pdb.prot091710

APA

Ekwall, K., & Thon, G. (2017). Spore analysis and tetrad dissection of Schizosaccharomyces pombe. Cold Spring Harbor Protocols, 2017(7), 541-544. https://doi.org/10.1101/pdb.prot091710

Vancouver

Ekwall K, Thon G. Spore analysis and tetrad dissection of Schizosaccharomyces pombe. Cold Spring Harbor Protocols. 2017 Jul;2017(7):541-544. https://doi.org/10.1101/pdb.prot091710

Author

Ekwall, Karl ; Thon, Genevieve. / Spore analysis and tetrad dissection of Schizosaccharomyces pombe. In: Cold Spring Harbor Protocols. 2017 ; Vol. 2017, No. 7. pp. 541-544.

Bibtex

@article{58a009271e8e4d2980563d01a8d4dcf5,
title = "Spore analysis and tetrad dissection of Schizosaccharomyces pombe",
abstract = "Here we describe the processing of Schizosaccharomyces pombe spores in batches (random spore analysis) or through tetrad dissections. Spores are usually prepared from matings between haploid strains (producing zygotic asci) or from sporulating diploids (producing azygotic asci). In random spore analysis, a snail enzyme preparation is used to digest the walls of asci to release free spores that are diluted and plated to form colonies. In tetrad dissection, a needle attached to a micromanipulator is used to pick asci and separate spores. Tetrad dissection has traditionally been the method of choice for genetic mapping and is very useful in the study of genetic interactions (e.g., suppressor analysis). It is also the preferred method for routine crosses because it ensures that every colony stems from a single spore. This can never be certain in random spore analysis.",
author = "Karl Ekwall and Genevieve Thon",
year = "2017",
month = jul,
doi = "10.1101/pdb.prot091710",
language = "English",
volume = "2017",
pages = "541--544",
journal = "Cold Spring Harbor Protocols",
issn = "1940-3402",
publisher = "Cold Spring Harbor Laboratory Press",
number = "7",

}

RIS

TY - JOUR

T1 - Spore analysis and tetrad dissection of Schizosaccharomyces pombe

AU - Ekwall, Karl

AU - Thon, Genevieve

PY - 2017/7

Y1 - 2017/7

N2 - Here we describe the processing of Schizosaccharomyces pombe spores in batches (random spore analysis) or through tetrad dissections. Spores are usually prepared from matings between haploid strains (producing zygotic asci) or from sporulating diploids (producing azygotic asci). In random spore analysis, a snail enzyme preparation is used to digest the walls of asci to release free spores that are diluted and plated to form colonies. In tetrad dissection, a needle attached to a micromanipulator is used to pick asci and separate spores. Tetrad dissection has traditionally been the method of choice for genetic mapping and is very useful in the study of genetic interactions (e.g., suppressor analysis). It is also the preferred method for routine crosses because it ensures that every colony stems from a single spore. This can never be certain in random spore analysis.

AB - Here we describe the processing of Schizosaccharomyces pombe spores in batches (random spore analysis) or through tetrad dissections. Spores are usually prepared from matings between haploid strains (producing zygotic asci) or from sporulating diploids (producing azygotic asci). In random spore analysis, a snail enzyme preparation is used to digest the walls of asci to release free spores that are diluted and plated to form colonies. In tetrad dissection, a needle attached to a micromanipulator is used to pick asci and separate spores. Tetrad dissection has traditionally been the method of choice for genetic mapping and is very useful in the study of genetic interactions (e.g., suppressor analysis). It is also the preferred method for routine crosses because it ensures that every colony stems from a single spore. This can never be certain in random spore analysis.

UR - http://www.scopus.com/inward/record.url?scp=85021765775&partnerID=8YFLogxK

U2 - 10.1101/pdb.prot091710

DO - 10.1101/pdb.prot091710

M3 - Journal article

C2 - 28679703

AN - SCOPUS:85021765775

VL - 2017

SP - 541

EP - 544

JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

SN - 1940-3402

IS - 7

ER -

ID: 181455762