Targeting synthesis of the Chromosome Replication Initiator Protein DnaA by antisense PNA-peptide conjugates in Escherichia coli

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Targeting synthesis of the Chromosome Replication Initiator Protein DnaA by antisense PNA-peptide conjugates in Escherichia coli. / Campion, Christopher; Charbon, Godefroid; Nielsen, Peter E.; Løbner-Olesen, Anders.

In: Frontiers in Antibiotics, Vol. 3, 1384390, 2024.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Campion, C, Charbon, G, Nielsen, PE & Løbner-Olesen, A 2024, 'Targeting synthesis of the Chromosome Replication Initiator Protein DnaA by antisense PNA-peptide conjugates in Escherichia coli', Frontiers in Antibiotics, vol. 3, 1384390. https://doi.org/10.3389/frabi.2024.1384390

APA

Campion, C., Charbon, G., Nielsen, P. E., & Løbner-Olesen, A. (2024). Targeting synthesis of the Chromosome Replication Initiator Protein DnaA by antisense PNA-peptide conjugates in Escherichia coli. Frontiers in Antibiotics, 3, [1384390]. https://doi.org/10.3389/frabi.2024.1384390

Vancouver

Campion C, Charbon G, Nielsen PE, Løbner-Olesen A. Targeting synthesis of the Chromosome Replication Initiator Protein DnaA by antisense PNA-peptide conjugates in Escherichia coli. Frontiers in Antibiotics. 2024;3. 1384390. https://doi.org/10.3389/frabi.2024.1384390

Author

Campion, Christopher ; Charbon, Godefroid ; Nielsen, Peter E. ; Løbner-Olesen, Anders. / Targeting synthesis of the Chromosome Replication Initiator Protein DnaA by antisense PNA-peptide conjugates in Escherichia coli. In: Frontiers in Antibiotics. 2024 ; Vol. 3.

Bibtex

@article{198b82556f824b8ab6147261971283c2,
title = "Targeting synthesis of the Chromosome Replication Initiator Protein DnaA by antisense PNA-peptide conjugates in Escherichia coli",
abstract = "Initiation of chromosome replication is an essential stage of the bacterial cell cycle that is controlled by the DnaA protein. With the aim of developing novel antimicrobials, we have targeted the initiation of DNA replication, using antisense peptide nucleic acids (PNAs), directed against DnaA translation. A series of anti-DnaA PNA conjugated to lysine-rich bacterial penetrating peptides (PNA-BPPs) were designed to block DnaA translation. These anti-DnaA PNA-BPPs inhibited growth of wild-type Escherichia coli cells at low micromolar concentrations, and cells exposed to anti-DnaA PNA-BPPs exhibited characteristic hallmarks of chromosome replication inhibition. These results present one of very few compounds successfully targeting initiation of chromosome replication, an essential step in the bacterial cell cycle.",
author = "Christopher Campion and Godefroid Charbon and Nielsen, {Peter E.} and Anders L{\o}bner-Olesen",
year = "2024",
doi = "10.3389/frabi.2024.1384390",
language = "English",
volume = "3",
journal = "Frontiers in Antibiotics",
issn = "2813-2467",
publisher = "Frontiers Media",

}

RIS

TY - JOUR

T1 - Targeting synthesis of the Chromosome Replication Initiator Protein DnaA by antisense PNA-peptide conjugates in Escherichia coli

AU - Campion, Christopher

AU - Charbon, Godefroid

AU - Nielsen, Peter E.

AU - Løbner-Olesen, Anders

PY - 2024

Y1 - 2024

N2 - Initiation of chromosome replication is an essential stage of the bacterial cell cycle that is controlled by the DnaA protein. With the aim of developing novel antimicrobials, we have targeted the initiation of DNA replication, using antisense peptide nucleic acids (PNAs), directed against DnaA translation. A series of anti-DnaA PNA conjugated to lysine-rich bacterial penetrating peptides (PNA-BPPs) were designed to block DnaA translation. These anti-DnaA PNA-BPPs inhibited growth of wild-type Escherichia coli cells at low micromolar concentrations, and cells exposed to anti-DnaA PNA-BPPs exhibited characteristic hallmarks of chromosome replication inhibition. These results present one of very few compounds successfully targeting initiation of chromosome replication, an essential step in the bacterial cell cycle.

AB - Initiation of chromosome replication is an essential stage of the bacterial cell cycle that is controlled by the DnaA protein. With the aim of developing novel antimicrobials, we have targeted the initiation of DNA replication, using antisense peptide nucleic acids (PNAs), directed against DnaA translation. A series of anti-DnaA PNA conjugated to lysine-rich bacterial penetrating peptides (PNA-BPPs) were designed to block DnaA translation. These anti-DnaA PNA-BPPs inhibited growth of wild-type Escherichia coli cells at low micromolar concentrations, and cells exposed to anti-DnaA PNA-BPPs exhibited characteristic hallmarks of chromosome replication inhibition. These results present one of very few compounds successfully targeting initiation of chromosome replication, an essential step in the bacterial cell cycle.

U2 - 10.3389/frabi.2024.1384390

DO - 10.3389/frabi.2024.1384390

M3 - Journal article

VL - 3

JO - Frontiers in Antibiotics

JF - Frontiers in Antibiotics

SN - 2813-2467

M1 - 1384390

ER -

ID: 387827057