Tawny Owls Strix aluco have been reported to skew the sex ratio of their offspring towards males when facing food shortage during the nestling period (and vice versa), because female fitness is more compromised by food shortage during development than male fitness. To test the generality of these results we used a DNA marker technique to determine the sex ratio in broods of Tawny Owls in Danish deciduous woodland during two years of ample food supply (rodent population outbreak) and two years of poor food supply. Of 268 nestlings, 59% were males (95% CI: 53-65%). This proportion was higher than previously reported for the species (49% in Northumberland, UK, and 52% in Hungary), but consistent with Fisherian sex allocation, which predicts a male bias of c. 57% based on inferred differences in energy requirements of male and female chicks. Contrary to previous results, brood sex ratios were not correlated with the resource abundance during the breeding seasons, despite considerable variation in breeding frequency, brood size or hatching date across years. Brood sex ratios were unaffected by brood reduction prior to DNA sampling, and nestling mortality rates after DNA sampling were not related to gender. The inconsistency between the sex ratio allocation patterns in our study and previous investigations suggests that adaptive sex allocation strategies differ across populations. These differences may relate to reproductive constraints in our population, where reproductive decisions seem primarily to concern whether to lay eggs at all, rather than adjust the sex ratio to differences in starvation risk of nestlings.