The A- and B-type muscarinic acetylcholine receptors from Drosophila melanogaster couple to different second messenger pathways
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The A- and B-type muscarinic acetylcholine receptors from Drosophila melanogaster couple to different second messenger pathways. / Ren, Guilin Robin; Folke, Jonas; Hauser, Frank; Li, Shizhong; Grimmelikhuijzen, Cornelis.
In: Biochemical and Biophysical Research Communications, Vol. 462, No. 4, 2015, p. 358-364.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - The A- and B-type muscarinic acetylcholine receptors from Drosophila melanogaster couple to different second messenger pathways
AU - Ren, Guilin Robin
AU - Folke, Jonas
AU - Hauser, Frank
AU - Li, Shizhong
AU - Grimmelikhuijzen, Cornelis
N1 - Copyright © 2015. Published by Elsevier Inc.
PY - 2015
Y1 - 2015
N2 - Muscarinic acetylcholine receptors (mAChRs) are G protein-coupled receptors (GPCRs) that are activated by the agonists acetylcholine and muscarine and blocked by several antagonists, among them atropine. In mammals five mAChRs (m1-m5) exist of which m1, m3, and m5 are coupled to members of the Gq/11 family and m2 and m4 to members of the Gi/0 family. We have recently shown that Drosophila melanogaster and other arthropods have two mAChRs, named A and B, where the A-type has the same pharmacology as the mammalian mAChRs, while the B-type has a very low affinity to muscarine and no affinity to classical antagonists such as atropine. Here, we find that the D. melanogaster A-type mAChR is coupled to Gq/11 and D. melanogaster B-type mAChR to Gi/0. Furthermore, by comparing the second and third intracellular loops of all animal mAChRs for which the G protein coupling has been established, we could identify several amino acid residues likely to be specific for either Gq/11 or Gi/0 coupling. Using these hallmarks for specific mAChR G protein interaction we found that all protostomes with a sequenced genome have one mAChR coupled to Gq/11 and one to four mAChRs coupled to Gi/0. Furthermore, in protostomes, probably all A-type mAChRs are coupled to Gq/11 and all B-type mAChRs to G0/i.
AB - Muscarinic acetylcholine receptors (mAChRs) are G protein-coupled receptors (GPCRs) that are activated by the agonists acetylcholine and muscarine and blocked by several antagonists, among them atropine. In mammals five mAChRs (m1-m5) exist of which m1, m3, and m5 are coupled to members of the Gq/11 family and m2 and m4 to members of the Gi/0 family. We have recently shown that Drosophila melanogaster and other arthropods have two mAChRs, named A and B, where the A-type has the same pharmacology as the mammalian mAChRs, while the B-type has a very low affinity to muscarine and no affinity to classical antagonists such as atropine. Here, we find that the D. melanogaster A-type mAChR is coupled to Gq/11 and D. melanogaster B-type mAChR to Gi/0. Furthermore, by comparing the second and third intracellular loops of all animal mAChRs for which the G protein coupling has been established, we could identify several amino acid residues likely to be specific for either Gq/11 or Gi/0 coupling. Using these hallmarks for specific mAChR G protein interaction we found that all protostomes with a sequenced genome have one mAChR coupled to Gq/11 and one to four mAChRs coupled to Gi/0. Furthermore, in protostomes, probably all A-type mAChRs are coupled to Gq/11 and all B-type mAChRs to G0/i.
U2 - 10.1016/j.bbrc.2015.04.141
DO - 10.1016/j.bbrc.2015.04.141
M3 - Journal article
C2 - 25964087
VL - 462
SP - 358
EP - 364
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -
ID: 137422818