The FASTQ+ format and PISA

Research output: Contribution to journalJournal articleResearchpeer-review

The FASTQ+ format is designed for single-cell experiments. It extends various optional tags, including cell barcodes and unique molecular identifiers, to the sequence identifier and is fully compatible with the FASTQ format. In addition, PISA implements various utilities for processing sequences in the FASTQ format and alignments in the SAM/ BAM/CRAMformat from single-cell experiments, such as converting FASTQ format to FASTQ+, annotating alignments, PCR deduplication, feature counting and barcodes correction. The software is open-source and written in C language.

Availability and implementation: or

Contact: or

Supplementary information: Supplementary data are available at Bioinformatics online.

Original languageEnglish
Issue number19
Pages (from-to)4639-4642
Number of pages4
Publication statusPublished - 2022

ID: 322871829