The mRNA decapping machinery targets LBD3/ASL9 to mediate apical hook and lateral root development
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The mRNA decapping machinery targets LBD3/ASL9 to mediate apical hook and lateral root development. / Zuo, Zhangli; Roux, Milena E.; Chevalier, Jonathan R.; Dagdas, Yasin F.; Yamashino, Takafumi; Højgaard, Søren D.; Knight, Emilie; Østergaard, Lars; Rodriguez, Eleazar; Petersen, Morten.
In: Life Science Alliance, Vol. 6, No. 9, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - The mRNA decapping machinery targets LBD3/ASL9 to mediate apical hook and lateral root development
AU - Zuo, Zhangli
AU - Roux, Milena E.
AU - Chevalier, Jonathan R.
AU - Dagdas, Yasin F.
AU - Yamashino, Takafumi
AU - Højgaard, Søren D.
AU - Knight, Emilie
AU - Østergaard, Lars
AU - Rodriguez, Eleazar
AU - Petersen, Morten
N1 - Publisher Copyright: © 2023 Zuo et al.
PY - 2023
Y1 - 2023
N2 - Multicellular organisms perceive and transduce multiple cues to optimize development. Key transcription factors drive developmental changes, but RNA processing also contributes to tissue development. Here, we report that multiple decapping deficient mutants share developmental defects in apical hook, primary and lateral root growth. More specifically, LATERAL ORGAN BOUNDARIES DOMAIN 3 (LBD3)/ASYMMETRIC LEAVES 2-LIKE 9 (ASL9) transcripts accumulate in decapping deficient plants and can be found in complexes with decapping components. Accumulation of ASL9 inhibits apical hook and lateral root formation. Interestingly, exogenous auxin application restores lateral roots formation in both ASL9 over-expressors and mRNA decay-deficient mutants. Likewise, mutations in the cytokinin transcription factors type-B ARABIDOPSIS RESPONSE REGULATORS (B-ARRs) ARR10 and ARR12 restore the developmental defects caused by over-accumulation of capped ASL9 transcript upon ASL9 overexpression. Most importantly, loss-of-function of asl9 partially restores apical hook and lateral root formation in both dcp5-1 and pat triple decapping deficient mutants. Thus, the mRNA decay machinery directly targets ASL9 transcripts for decay, possibly to interfere with cytokinin/auxin responses, during development.
AB - Multicellular organisms perceive and transduce multiple cues to optimize development. Key transcription factors drive developmental changes, but RNA processing also contributes to tissue development. Here, we report that multiple decapping deficient mutants share developmental defects in apical hook, primary and lateral root growth. More specifically, LATERAL ORGAN BOUNDARIES DOMAIN 3 (LBD3)/ASYMMETRIC LEAVES 2-LIKE 9 (ASL9) transcripts accumulate in decapping deficient plants and can be found in complexes with decapping components. Accumulation of ASL9 inhibits apical hook and lateral root formation. Interestingly, exogenous auxin application restores lateral roots formation in both ASL9 over-expressors and mRNA decay-deficient mutants. Likewise, mutations in the cytokinin transcription factors type-B ARABIDOPSIS RESPONSE REGULATORS (B-ARRs) ARR10 and ARR12 restore the developmental defects caused by over-accumulation of capped ASL9 transcript upon ASL9 overexpression. Most importantly, loss-of-function of asl9 partially restores apical hook and lateral root formation in both dcp5-1 and pat triple decapping deficient mutants. Thus, the mRNA decay machinery directly targets ASL9 transcripts for decay, possibly to interfere with cytokinin/auxin responses, during development.
U2 - 10.26508/lsa.202302090
DO - 10.26508/lsa.202302090
M3 - Journal article
C2 - 37385753
AN - SCOPUS:85164230606
VL - 6
JO - Life Science Alliance
JF - Life Science Alliance
SN - 2575-1077
IS - 9
ER -
ID: 359722771