The Na+/H+ exchanger NHE1, but not the Na+, HCO3- cotransporter NBCn1, regulates motility of MCF7 breast cancer cells expressing constitutively active ErbB2

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The Na+/H+ exchanger NHE1, but not the Na+, HCO3- cotransporter NBCn1, regulates motility of MCF7 breast cancer cells expressing constitutively active ErbB2. / Lauritzen, Gitte; Stock, Christian-Martin; Lemaire, Justine; Lund, Stine F.; Jensen, Mie Frid; Damsgaard, Britt; Petersen, Katrine Seide; Wiwel, Maria; Rønnov-Jessen, Lone; Schwab, Albrecht; Pedersen, Stine Helene Falsig.

In: Cancer Letters, Vol. 317, No. 2, 2012, p. 172-183.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Lauritzen, G, Stock, C-M, Lemaire, J, Lund, SF, Jensen, MF, Damsgaard, B, Petersen, KS, Wiwel, M, Rønnov-Jessen, L, Schwab, A & Pedersen, SHF 2012, 'The Na+/H+ exchanger NHE1, but not the Na+, HCO3- cotransporter NBCn1, regulates motility of MCF7 breast cancer cells expressing constitutively active ErbB2', Cancer Letters, vol. 317, no. 2, pp. 172-183. https://doi.org/10.1016/j.canlet.2011.11.023

APA

Lauritzen, G., Stock, C-M., Lemaire, J., Lund, S. F., Jensen, M. F., Damsgaard, B., Petersen, K. S., Wiwel, M., Rønnov-Jessen, L., Schwab, A., & Pedersen, S. H. F. (2012). The Na+/H+ exchanger NHE1, but not the Na+, HCO3- cotransporter NBCn1, regulates motility of MCF7 breast cancer cells expressing constitutively active ErbB2. Cancer Letters, 317(2), 172-183. https://doi.org/10.1016/j.canlet.2011.11.023

Vancouver

Lauritzen G, Stock C-M, Lemaire J, Lund SF, Jensen MF, Damsgaard B et al. The Na+/H+ exchanger NHE1, but not the Na+, HCO3- cotransporter NBCn1, regulates motility of MCF7 breast cancer cells expressing constitutively active ErbB2. Cancer Letters. 2012;317(2):172-183. https://doi.org/10.1016/j.canlet.2011.11.023

Author

Lauritzen, Gitte ; Stock, Christian-Martin ; Lemaire, Justine ; Lund, Stine F. ; Jensen, Mie Frid ; Damsgaard, Britt ; Petersen, Katrine Seide ; Wiwel, Maria ; Rønnov-Jessen, Lone ; Schwab, Albrecht ; Pedersen, Stine Helene Falsig. / The Na+/H+ exchanger NHE1, but not the Na+, HCO3- cotransporter NBCn1, regulates motility of MCF7 breast cancer cells expressing constitutively active ErbB2. In: Cancer Letters. 2012 ; Vol. 317, No. 2. pp. 172-183.

Bibtex

@article{ce5026d808c24d80ba6580b638e25350,
title = "The Na+/H+ exchanger NHE1, but not the Na+, HCO3- cotransporter NBCn1, regulates motility of MCF7 breast cancer cells expressing constitutively active ErbB2",
abstract = "We and others have shown central roles of the Na(+)/H(+) exchanger NHE1 in cell motility. The aim of this study was to determine the roles of NHE1 and of the Na(+), HCO(3)(-) cotransporter NBCn1 in motility of serum-starved MCF-7 breast cancer cells expressing constitutively active ErbB2 (¿NErbB2). ¿NErbB2 expression elicited NBCn1 upregulation, Ser(703)-phosphorylation of NHE1, and NHE1-inhibitor (EIPA)-sensitive pericellular acidification, in conjunction with increased expression of {\ss}1 integrin and ERM proteins. Active ERM proteins and NHE1 colocalized strongly to invadopodial rosettes, the diameter of which was increased by ¿NErbB2. Adhesion and migration on collagen-I were augmented by ¿NErbB2, unaffected by the NBC inhibitor S0859, and further stimulated by EIPA in a manner potentiated by PI3K-Akt-inhibition. These findings demonstrate that NHE1 inhibition can enhance cancer cell motility, adding an important facet to the understanding of NHE1 in cancer.",
author = "Gitte Lauritzen and Christian-Martin Stock and Justine Lemaire and Lund, {Stine F.} and Jensen, {Mie Frid} and Britt Damsgaard and Petersen, {Katrine Seide} and Maria Wiwel and Lone R{\o}nnov-Jessen and Albrecht Schwab and Pedersen, {Stine Helene Falsig}",
note = "Copyright {\textcopyright} 2011 Elsevier Ireland Ltd. All rights reserved.",
year = "2012",
doi = "10.1016/j.canlet.2011.11.023",
language = "English",
volume = "317",
pages = "172--183",
journal = "Cancer Letters",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

RIS

TY - JOUR

T1 - The Na+/H+ exchanger NHE1, but not the Na+, HCO3- cotransporter NBCn1, regulates motility of MCF7 breast cancer cells expressing constitutively active ErbB2

AU - Lauritzen, Gitte

AU - Stock, Christian-Martin

AU - Lemaire, Justine

AU - Lund, Stine F.

AU - Jensen, Mie Frid

AU - Damsgaard, Britt

AU - Petersen, Katrine Seide

AU - Wiwel, Maria

AU - Rønnov-Jessen, Lone

AU - Schwab, Albrecht

AU - Pedersen, Stine Helene Falsig

N1 - Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

PY - 2012

Y1 - 2012

N2 - We and others have shown central roles of the Na(+)/H(+) exchanger NHE1 in cell motility. The aim of this study was to determine the roles of NHE1 and of the Na(+), HCO(3)(-) cotransporter NBCn1 in motility of serum-starved MCF-7 breast cancer cells expressing constitutively active ErbB2 (¿NErbB2). ¿NErbB2 expression elicited NBCn1 upregulation, Ser(703)-phosphorylation of NHE1, and NHE1-inhibitor (EIPA)-sensitive pericellular acidification, in conjunction with increased expression of ß1 integrin and ERM proteins. Active ERM proteins and NHE1 colocalized strongly to invadopodial rosettes, the diameter of which was increased by ¿NErbB2. Adhesion and migration on collagen-I were augmented by ¿NErbB2, unaffected by the NBC inhibitor S0859, and further stimulated by EIPA in a manner potentiated by PI3K-Akt-inhibition. These findings demonstrate that NHE1 inhibition can enhance cancer cell motility, adding an important facet to the understanding of NHE1 in cancer.

AB - We and others have shown central roles of the Na(+)/H(+) exchanger NHE1 in cell motility. The aim of this study was to determine the roles of NHE1 and of the Na(+), HCO(3)(-) cotransporter NBCn1 in motility of serum-starved MCF-7 breast cancer cells expressing constitutively active ErbB2 (¿NErbB2). ¿NErbB2 expression elicited NBCn1 upregulation, Ser(703)-phosphorylation of NHE1, and NHE1-inhibitor (EIPA)-sensitive pericellular acidification, in conjunction with increased expression of ß1 integrin and ERM proteins. Active ERM proteins and NHE1 colocalized strongly to invadopodial rosettes, the diameter of which was increased by ¿NErbB2. Adhesion and migration on collagen-I were augmented by ¿NErbB2, unaffected by the NBC inhibitor S0859, and further stimulated by EIPA in a manner potentiated by PI3K-Akt-inhibition. These findings demonstrate that NHE1 inhibition can enhance cancer cell motility, adding an important facet to the understanding of NHE1 in cancer.

U2 - 10.1016/j.canlet.2011.11.023

DO - 10.1016/j.canlet.2011.11.023

M3 - Journal article

C2 - 22120673

VL - 317

SP - 172

EP - 183

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 2

ER -

ID: 37624261