Three Ribosomal Operons of Escherichia coli Contain Genes Encoding Small RNAs That Interact With Hfq and CsrA in vitro
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Three Ribosomal Operons of Escherichia coli Contain Genes Encoding Small RNAs That Interact With Hfq and CsrA in vitro. / Stenum, Thomas Søndergaard; Kongstad, Mette; Holmqvist, Erik; Kallipolitis, Birgitte; Svenningsen, Sine Lo; Sørensen, Michael Askvad.
In: Frontiers in Microbiology, Vol. 12, 625585, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Three Ribosomal Operons of Escherichia coli Contain Genes Encoding Small RNAs That Interact With Hfq and CsrA in vitro
AU - Stenum, Thomas Søndergaard
AU - Kongstad, Mette
AU - Holmqvist, Erik
AU - Kallipolitis, Birgitte
AU - Svenningsen, Sine Lo
AU - Sørensen, Michael Askvad
N1 - Publisher Copyright: © Copyright © 2021 Stenum, Kongstad, Holmqvist, Kallipolitis, Svenningsen and Sørensen.
PY - 2021
Y1 - 2021
N2 - Three out of the seven ribosomal RNA operons in Escherichia coli end in dual terminator structures. Between the two terminators of each operon is a short sequence that we report here to be an sRNA gene, transcribed as part of the ribosomal RNA primary transcript by read-through of the first terminator. The sRNA genes (rrA, rrB and rrF) from the three operons (rrnA, rrnB and rrnD) are more than 98% identical, and pull-down experiments show that their transcripts interact with Hfq and CsrA. Deletion of rrA, B, F, as well as overexpression of rrB, only modestly affect known CsrA-regulated phenotypes like biofilm formation, pgaA translation and glgC translation, and the role of the sRNAs in vivo may not yet be fully understood. Since RrA, B, F are short-lived and transcribed along with the ribosomal RNA components, their concentration reflect growth-rate regulation at the ribosomal RNA promoters and they could function to fine-tune other growth-phase-dependent processes in the cell. The primary and secondary structure of these small RNAs are conserved among species belonging to different genera of Enterobacteriales.
AB - Three out of the seven ribosomal RNA operons in Escherichia coli end in dual terminator structures. Between the two terminators of each operon is a short sequence that we report here to be an sRNA gene, transcribed as part of the ribosomal RNA primary transcript by read-through of the first terminator. The sRNA genes (rrA, rrB and rrF) from the three operons (rrnA, rrnB and rrnD) are more than 98% identical, and pull-down experiments show that their transcripts interact with Hfq and CsrA. Deletion of rrA, B, F, as well as overexpression of rrB, only modestly affect known CsrA-regulated phenotypes like biofilm formation, pgaA translation and glgC translation, and the role of the sRNAs in vivo may not yet be fully understood. Since RrA, B, F are short-lived and transcribed along with the ribosomal RNA components, their concentration reflect growth-rate regulation at the ribosomal RNA promoters and they could function to fine-tune other growth-phase-dependent processes in the cell. The primary and secondary structure of these small RNAs are conserved among species belonging to different genera of Enterobacteriales.
KW - CsrA
KW - dual terminators
KW - Hfq
KW - ribosomal RNA operon
KW - sRNA
U2 - 10.3389/fmicb.2021.625585
DO - 10.3389/fmicb.2021.625585
M3 - Journal article
C2 - 34046019
AN - SCOPUS:85107073787
VL - 12
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
SN - 1664-302X
M1 - 625585
ER -
ID: 272015192