Ultrabright planar optodes for luminescence life-time based microscopic imaging of O2 dynamics in biofilms
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Ultrabright planar optodes for luminescence life-time based microscopic imaging of O2 dynamics in biofilms. / Staal, Marc Jaap; Borisov, S M; Rickelt, L F; Klimant, I; Kühl, M.
In: Journal of Microbiological Methods, Vol. 85, No. 1, 04.2011, p. 67-74.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Ultrabright planar optodes for luminescence life-time based microscopic imaging of O2 dynamics in biofilms
AU - Staal, Marc Jaap
AU - Borisov, S M
AU - Rickelt, L F
AU - Klimant, I
AU - Kühl, M
N1 - Copyright © 2010 Elsevier B.V. All rights reserved.
PY - 2011/4
Y1 - 2011/4
N2 - New transparent optodes for life-time based microscopic imaging of O2 were developed by spin-coating a µm-thin layer of a highly luminescent cyclometalated iridium(III) coumarin complex in polystyrene onto glass cover slips. Compared to similar thin-film O2 optodes based on a ruthenium(II) polypyridyl complex or a platinum(II) porphyrin, the new planar sensors have i) higher brightness allowing for much shorter exposure times and thus higher time resolution, ii) more homogeneous and smaller pixel to pixel variation over the sensor area resulting in less noisy O2 images, and iii) a lower temperature dependency simplifying calibration procedures. We used the new optodes for microscopic imaging of the spatio-temporal O2 dynamics at the base of heterotrophic biofilms in combination with confocal imaging of bacterial biomass and biofilm structure. This allowed us to directly link biomass distribution to O2 distribution under both steady state and non-steady state conditions. We demonstrate that the O2 dynamics in biofilms is governed by a complex interaction between biomass distribution, mass transfer and flow that cannot be directly inferred from structural information on biomass distribution alone.
AB - New transparent optodes for life-time based microscopic imaging of O2 were developed by spin-coating a µm-thin layer of a highly luminescent cyclometalated iridium(III) coumarin complex in polystyrene onto glass cover slips. Compared to similar thin-film O2 optodes based on a ruthenium(II) polypyridyl complex or a platinum(II) porphyrin, the new planar sensors have i) higher brightness allowing for much shorter exposure times and thus higher time resolution, ii) more homogeneous and smaller pixel to pixel variation over the sensor area resulting in less noisy O2 images, and iii) a lower temperature dependency simplifying calibration procedures. We used the new optodes for microscopic imaging of the spatio-temporal O2 dynamics at the base of heterotrophic biofilms in combination with confocal imaging of bacterial biomass and biofilm structure. This allowed us to directly link biomass distribution to O2 distribution under both steady state and non-steady state conditions. We demonstrate that the O2 dynamics in biofilms is governed by a complex interaction between biomass distribution, mass transfer and flow that cannot be directly inferred from structural information on biomass distribution alone.
KW - Bacteria
KW - Biofilms
KW - Biomass
KW - Luminescent Measurements
KW - Microscopy
KW - Oxygen
U2 - 10.1016/j.mimet.2011.01.021
DO - 10.1016/j.mimet.2011.01.021
M3 - Journal article
C2 - 21277912
VL - 85
SP - 67
EP - 74
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
SN - 0167-7012
IS - 1
ER -
ID: 37367957