Use of a Whole-Cell Biosensor and Flow Cytometry to Detect AHL Production by an Indigenous Soil Community During Decomposition

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Use of a Whole-Cell Biosensor and Flow Cytometry to Detect AHL Production by an Indigenous Soil Community During Decomposition. / Burmølle, Mette; Hansen, Lars Hestbjerg; Sørensen, Søren Johannes.

In: Microbial Ecology, Vol. 50, No. 2, 2005, p. 221-229.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Burmølle, M, Hansen, LH & Sørensen, SJ 2005, 'Use of a Whole-Cell Biosensor and Flow Cytometry to Detect AHL Production by an Indigenous Soil Community During Decomposition', Microbial Ecology, vol. 50, no. 2, pp. 221-229. https://doi.org/10.1007/s00248-004-0113-8

APA

Burmølle, M., Hansen, L. H., & Sørensen, S. J. (2005). Use of a Whole-Cell Biosensor and Flow Cytometry to Detect AHL Production by an Indigenous Soil Community During Decomposition. Microbial Ecology, 50(2), 221-229. https://doi.org/10.1007/s00248-004-0113-8

Vancouver

Burmølle M, Hansen LH, Sørensen SJ. Use of a Whole-Cell Biosensor and Flow Cytometry to Detect AHL Production by an Indigenous Soil Community During Decomposition. Microbial Ecology. 2005;50(2):221-229. https://doi.org/10.1007/s00248-004-0113-8

Author

Burmølle, Mette ; Hansen, Lars Hestbjerg ; Sørensen, Søren Johannes. / Use of a Whole-Cell Biosensor and Flow Cytometry to Detect AHL Production by an Indigenous Soil Community During Decomposition. In: Microbial Ecology. 2005 ; Vol. 50, No. 2. pp. 221-229.

Bibtex

@article{3789bce074c311dbbee902004c4f4f50,
title = "Use of a Whole-Cell Biosensor and Flow Cytometry to Detect AHL Production by an Indigenous Soil Community During Decomposition",
abstract = "Quorum sensing, mediated by acylated homoserine lactones (AHLs), is well described for pure culture bacteria, but few studies report detection of AHL compounds in natural bacterial habitats. In this study, we detect AHL production during a degradation process in soil by use of whole-cell biosensor technology and flow cytometry analysis. An indigenous soil bacterium, belonging to the family of Enterobacteriaceae, was isolated and transformed with a low-copy plasmid harboring a gene encoding an unstable variant of the green fluorescent protein (gfpASV) fused to the AHL-regulated PluxI promoter originating from Vibrio fischeri. This resulted in a whole-cell biosensor, responding to the presence of AHL compounds. The biosensor was introduced to compost soil microcosms amended with nettle leaves. After 3 days of incubation, cells were extracted and analyzed by flow cytometry. All microcosms contained induced biosensors. From these microcosms, AHL producers were isolated and further identified as species previously shown to produce AHLs. The results demonstrate that AHL compounds are produced during degradation of litter in soil, indicating the presence of AHL-mediated quorum sensing in this environment.",
author = "Mette Burm{\o}lle and Hansen, {Lars Hestbjerg} and S{\o}rensen, {S{\o}ren Johannes}",
year = "2005",
doi = "10.1007/s00248-004-0113-8",
language = "English",
volume = "50",
pages = "221--229",
journal = "Microbial Ecology",
issn = "0095-3628",
publisher = "Springer",
number = "2",

}

RIS

TY - JOUR

T1 - Use of a Whole-Cell Biosensor and Flow Cytometry to Detect AHL Production by an Indigenous Soil Community During Decomposition

AU - Burmølle, Mette

AU - Hansen, Lars Hestbjerg

AU - Sørensen, Søren Johannes

PY - 2005

Y1 - 2005

N2 - Quorum sensing, mediated by acylated homoserine lactones (AHLs), is well described for pure culture bacteria, but few studies report detection of AHL compounds in natural bacterial habitats. In this study, we detect AHL production during a degradation process in soil by use of whole-cell biosensor technology and flow cytometry analysis. An indigenous soil bacterium, belonging to the family of Enterobacteriaceae, was isolated and transformed with a low-copy plasmid harboring a gene encoding an unstable variant of the green fluorescent protein (gfpASV) fused to the AHL-regulated PluxI promoter originating from Vibrio fischeri. This resulted in a whole-cell biosensor, responding to the presence of AHL compounds. The biosensor was introduced to compost soil microcosms amended with nettle leaves. After 3 days of incubation, cells were extracted and analyzed by flow cytometry. All microcosms contained induced biosensors. From these microcosms, AHL producers were isolated and further identified as species previously shown to produce AHLs. The results demonstrate that AHL compounds are produced during degradation of litter in soil, indicating the presence of AHL-mediated quorum sensing in this environment.

AB - Quorum sensing, mediated by acylated homoserine lactones (AHLs), is well described for pure culture bacteria, but few studies report detection of AHL compounds in natural bacterial habitats. In this study, we detect AHL production during a degradation process in soil by use of whole-cell biosensor technology and flow cytometry analysis. An indigenous soil bacterium, belonging to the family of Enterobacteriaceae, was isolated and transformed with a low-copy plasmid harboring a gene encoding an unstable variant of the green fluorescent protein (gfpASV) fused to the AHL-regulated PluxI promoter originating from Vibrio fischeri. This resulted in a whole-cell biosensor, responding to the presence of AHL compounds. The biosensor was introduced to compost soil microcosms amended with nettle leaves. After 3 days of incubation, cells were extracted and analyzed by flow cytometry. All microcosms contained induced biosensors. From these microcosms, AHL producers were isolated and further identified as species previously shown to produce AHLs. The results demonstrate that AHL compounds are produced during degradation of litter in soil, indicating the presence of AHL-mediated quorum sensing in this environment.

U2 - 10.1007/s00248-004-0113-8

DO - 10.1007/s00248-004-0113-8

M3 - Journal article

C2 - 16195831

VL - 50

SP - 221

EP - 229

JO - Microbial Ecology

JF - Microbial Ecology

SN - 0095-3628

IS - 2

ER -

ID: 88645