Use of laminar flow patterning for miniaturised biochemical assays

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Use of laminar flow patterning for miniaturised biochemical assays. / Regenberg, Birgitte; Krühne, Ulrich; Beyer, Michael; Pedersen, Lars H.; Simón, Marcos; Thomas, Owen R.T.; Nielsen, Jens; Ahl, Thomas.

In: Lab on a Chip, Vol. 4, No. 6, 12.2004, p. 654-657.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Regenberg, B, Krühne, U, Beyer, M, Pedersen, LH, Simón, M, Thomas, ORT, Nielsen, J & Ahl, T 2004, 'Use of laminar flow patterning for miniaturised biochemical assays', Lab on a Chip, vol. 4, no. 6, pp. 654-657. https://doi.org/10.1039/b409141h

APA

Regenberg, B., Krühne, U., Beyer, M., Pedersen, L. H., Simón, M., Thomas, O. R. T., Nielsen, J., & Ahl, T. (2004). Use of laminar flow patterning for miniaturised biochemical assays. Lab on a Chip, 4(6), 654-657. https://doi.org/10.1039/b409141h

Vancouver

Regenberg B, Krühne U, Beyer M, Pedersen LH, Simón M, Thomas ORT et al. Use of laminar flow patterning for miniaturised biochemical assays. Lab on a Chip. 2004 Dec;4(6):654-657. https://doi.org/10.1039/b409141h

Author

Regenberg, Birgitte ; Krühne, Ulrich ; Beyer, Michael ; Pedersen, Lars H. ; Simón, Marcos ; Thomas, Owen R.T. ; Nielsen, Jens ; Ahl, Thomas. / Use of laminar flow patterning for miniaturised biochemical assays. In: Lab on a Chip. 2004 ; Vol. 4, No. 6. pp. 654-657.

Bibtex

@article{cf344752dfc54ddd9de1fac3f85d9da1,
title = "Use of laminar flow patterning for miniaturised biochemical assays",
abstract = "Laminar flow in microfluidic chambers was used to construct low (one dimensional) density arrays suitable for miniaturized biochemical assays. By varying the ratio of flows of two guiding streams flanking a sample stream, precise focusing and positioning of the latter was achieved, and reactive species carried in the sample stream were deposited on functionalized chip surfaces as discrete 50 μm wide lanes. Using different model systems we have confirmed the method's suitability for qualitative screening and quantification tasks in receptor-ligand assays, recording biotin-streptavidin interactions, DNA-hybridization and DNA-triplex formation. The system is simple, fast, reproducible, flexible, and has small sample requirements.",
author = "Birgitte Regenberg and Ulrich Kr{\"u}hne and Michael Beyer and Pedersen, {Lars H.} and Marcos Sim{\'o}n and Thomas, {Owen R.T.} and Jens Nielsen and Thomas Ahl",
year = "2004",
month = dec,
doi = "10.1039/b409141h",
language = "English",
volume = "4",
pages = "654--657",
journal = "Lab on a Chip",
issn = "1473-0197",
publisher = "Royal Society of Chemistry",
number = "6",

}

RIS

TY - JOUR

T1 - Use of laminar flow patterning for miniaturised biochemical assays

AU - Regenberg, Birgitte

AU - Krühne, Ulrich

AU - Beyer, Michael

AU - Pedersen, Lars H.

AU - Simón, Marcos

AU - Thomas, Owen R.T.

AU - Nielsen, Jens

AU - Ahl, Thomas

PY - 2004/12

Y1 - 2004/12

N2 - Laminar flow in microfluidic chambers was used to construct low (one dimensional) density arrays suitable for miniaturized biochemical assays. By varying the ratio of flows of two guiding streams flanking a sample stream, precise focusing and positioning of the latter was achieved, and reactive species carried in the sample stream were deposited on functionalized chip surfaces as discrete 50 μm wide lanes. Using different model systems we have confirmed the method's suitability for qualitative screening and quantification tasks in receptor-ligand assays, recording biotin-streptavidin interactions, DNA-hybridization and DNA-triplex formation. The system is simple, fast, reproducible, flexible, and has small sample requirements.

AB - Laminar flow in microfluidic chambers was used to construct low (one dimensional) density arrays suitable for miniaturized biochemical assays. By varying the ratio of flows of two guiding streams flanking a sample stream, precise focusing and positioning of the latter was achieved, and reactive species carried in the sample stream were deposited on functionalized chip surfaces as discrete 50 μm wide lanes. Using different model systems we have confirmed the method's suitability for qualitative screening and quantification tasks in receptor-ligand assays, recording biotin-streptavidin interactions, DNA-hybridization and DNA-triplex formation. The system is simple, fast, reproducible, flexible, and has small sample requirements.

UR - http://www.scopus.com/inward/record.url?scp=10644282362&partnerID=8YFLogxK

U2 - 10.1039/b409141h

DO - 10.1039/b409141h

M3 - Journal article

C2 - 15570380

AN - SCOPUS:10644282362

VL - 4

SP - 654

EP - 657

JO - Lab on a Chip

JF - Lab on a Chip

SN - 1473-0197

IS - 6

ER -

ID: 239905466