Where have all the Na+ channels gone? In search of functional ENaC in exocrine pancreas.

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Where have all the Na+ channels gone? In search of functional ENaC in exocrine pancreas. / Novak, Ivana; Hansen, Mette R.

In: BBA General Subjects, Vol. 1566, No. 1-2, 2002, p. 162-8.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Novak, I & Hansen, MR 2002, 'Where have all the Na+ channels gone? In search of functional ENaC in exocrine pancreas.', BBA General Subjects, vol. 1566, no. 1-2, pp. 162-8. https://doi.org/10.1016/S0005-2736(02)00598-9

APA

Novak, I., & Hansen, M. R. (2002). Where have all the Na+ channels gone? In search of functional ENaC in exocrine pancreas. BBA General Subjects, 1566(1-2), 162-8. https://doi.org/10.1016/S0005-2736(02)00598-9

Vancouver

Novak I, Hansen MR. Where have all the Na+ channels gone? In search of functional ENaC in exocrine pancreas. BBA General Subjects. 2002;1566(1-2):162-8. https://doi.org/10.1016/S0005-2736(02)00598-9

Author

Novak, Ivana ; Hansen, Mette R. / Where have all the Na+ channels gone? In search of functional ENaC in exocrine pancreas. In: BBA General Subjects. 2002 ; Vol. 1566, No. 1-2. pp. 162-8.

Bibtex

@article{46f3dfd0b18411ddb04f000ea68e967b,
title = "Where have all the Na+ channels gone? In search of functional ENaC in exocrine pancreas.",
abstract = "Many epithelia express specific Na(+) channels (ENaC) together with the cystic fibrosis regulator (CFTR) Cl(-) channels. Pancreatic ducts secrete HCO(3)(-)-rich fluid and express CFTR. However, the question whether they possess ENaC has not been consistently addressed. The aim of the present study was to investigate if pancreatic ducts express functional ENaC. Membrane voltages (V) of ducts isolated from rat pancreas were measured with microelectrodes or whole-cell patch-clamp technique. Amiloride and benzamil given from bath or luminal sides did not hyperpolarize V. Lowering of extracellular Na(+) concentrations had effects that were not consistent with a simple Na(+) conductance, but rather with a Na(+)/Ca(2+) exchange. Acute or long-lasting treatment of pancreatic ducts with mineralocorticoids had no effect on V of unstimulated or secretin-stimulated preparations. Furthermore, pre-treatment of animals with glucocorticoids had no effect on pancreatic fluid secretion evoked from ducts, or from acini. Hence, our study shows that pancreas especially pancreatic ducts do not express functional ENaC.",
author = "Ivana Novak and Hansen, {Mette R}",
note = "Keywords: Amiloride; Animals; Epithelial Sodium Channel; Female; Glucocorticoids; Membrane Potentials; Microelectrodes; Mineralocorticoids; Pancreas; Pancreatic Ducts; Patch-Clamp Techniques; Perfusion; Rats; Rats, Wistar; Secretin; Sincalide; Sodium; Sodium Channels",
year = "2002",
doi = "10.1016/S0005-2736(02)00598-9",
language = "English",
volume = "1566",
pages = "162--8",
journal = "B B A - General Subjects",
issn = "0304-4165",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Where have all the Na+ channels gone? In search of functional ENaC in exocrine pancreas.

AU - Novak, Ivana

AU - Hansen, Mette R

N1 - Keywords: Amiloride; Animals; Epithelial Sodium Channel; Female; Glucocorticoids; Membrane Potentials; Microelectrodes; Mineralocorticoids; Pancreas; Pancreatic Ducts; Patch-Clamp Techniques; Perfusion; Rats; Rats, Wistar; Secretin; Sincalide; Sodium; Sodium Channels

PY - 2002

Y1 - 2002

N2 - Many epithelia express specific Na(+) channels (ENaC) together with the cystic fibrosis regulator (CFTR) Cl(-) channels. Pancreatic ducts secrete HCO(3)(-)-rich fluid and express CFTR. However, the question whether they possess ENaC has not been consistently addressed. The aim of the present study was to investigate if pancreatic ducts express functional ENaC. Membrane voltages (V) of ducts isolated from rat pancreas were measured with microelectrodes or whole-cell patch-clamp technique. Amiloride and benzamil given from bath or luminal sides did not hyperpolarize V. Lowering of extracellular Na(+) concentrations had effects that were not consistent with a simple Na(+) conductance, but rather with a Na(+)/Ca(2+) exchange. Acute or long-lasting treatment of pancreatic ducts with mineralocorticoids had no effect on V of unstimulated or secretin-stimulated preparations. Furthermore, pre-treatment of animals with glucocorticoids had no effect on pancreatic fluid secretion evoked from ducts, or from acini. Hence, our study shows that pancreas especially pancreatic ducts do not express functional ENaC.

AB - Many epithelia express specific Na(+) channels (ENaC) together with the cystic fibrosis regulator (CFTR) Cl(-) channels. Pancreatic ducts secrete HCO(3)(-)-rich fluid and express CFTR. However, the question whether they possess ENaC has not been consistently addressed. The aim of the present study was to investigate if pancreatic ducts express functional ENaC. Membrane voltages (V) of ducts isolated from rat pancreas were measured with microelectrodes or whole-cell patch-clamp technique. Amiloride and benzamil given from bath or luminal sides did not hyperpolarize V. Lowering of extracellular Na(+) concentrations had effects that were not consistent with a simple Na(+) conductance, but rather with a Na(+)/Ca(2+) exchange. Acute or long-lasting treatment of pancreatic ducts with mineralocorticoids had no effect on V of unstimulated or secretin-stimulated preparations. Furthermore, pre-treatment of animals with glucocorticoids had no effect on pancreatic fluid secretion evoked from ducts, or from acini. Hence, our study shows that pancreas especially pancreatic ducts do not express functional ENaC.

U2 - 10.1016/S0005-2736(02)00598-9

DO - 10.1016/S0005-2736(02)00598-9

M3 - Journal article

C2 - 12421547

VL - 1566

SP - 162

EP - 168

JO - B B A - General Subjects

JF - B B A - General Subjects

SN - 0304-4165

IS - 1-2

ER -

ID: 8569867