Widespread premature transcription termination of Arabidopsis thaliana NLR genes by the spen protein FPA

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Widespread premature transcription termination of Arabidopsis thaliana NLR genes by the spen protein FPA. / Parker, Matthew T.; Knop, Katarzyna; Zacharaki, Vasiliki; Sherwood, Anna; Tomé, Daniel; Yu, Xuhong ; Martin, Pascal; Beynon, Jim; Michaels, Scott; Barton, Geoffrey; Simpson, Gordon G.

In: eLife, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Parker, MT, Knop, K, Zacharaki, V, Sherwood, A, Tomé, D, Yu, X, Martin, P, Beynon, J, Michaels, S, Barton, G & Simpson, GG 2021, 'Widespread premature transcription termination of Arabidopsis thaliana NLR genes by the spen protein FPA', eLife. https://doi.org/10.7554/ELIFE.65537

APA

Parker, M. T., Knop, K., Zacharaki, V., Sherwood, A., Tomé, D., Yu, X., Martin, P., Beynon, J., Michaels, S., Barton, G., & Simpson, G. G. (2021). Widespread premature transcription termination of Arabidopsis thaliana NLR genes by the spen protein FPA. eLife. https://doi.org/10.7554/ELIFE.65537

Vancouver

Parker MT, Knop K, Zacharaki V, Sherwood A, Tomé D, Yu X et al. Widespread premature transcription termination of Arabidopsis thaliana NLR genes by the spen protein FPA. eLife. 2021. https://doi.org/10.7554/ELIFE.65537

Author

Parker, Matthew T. ; Knop, Katarzyna ; Zacharaki, Vasiliki ; Sherwood, Anna ; Tomé, Daniel ; Yu, Xuhong ; Martin, Pascal ; Beynon, Jim ; Michaels, Scott ; Barton, Geoffrey ; Simpson, Gordon G. / Widespread premature transcription termination of Arabidopsis thaliana NLR genes by the spen protein FPA. In: eLife. 2021.

Bibtex

@article{40777ca3c220468a9072311f17e075df,
title = "Widespread premature transcription termination of Arabidopsis thaliana NLR genes by the spen protein FPA",
abstract = "Genes involved in disease resistance are some of the fastest evolving and most diverse components of genomes. Large numbers of nucleotide-binding, leucine-rich repeat (NLR) genes are found in plant genomes and are required for disease resistance. However, NLRs can trigger autoimmunity, disrupt beneficial microbiota or reduce fitness. It is therefore crucial to understand how NLRs are controlled. Here, we show that the RNA-binding protein FPA mediates widespread premature cleavage and polyadenylation of NLR transcripts, thereby controlling their functional expression and impacting immunity. Using long-read Nanopore direct RNA sequencing, we resolved the complexity of NLR transcript processing and gene annotation. Our results uncover a co-transcriptional layer of NLR control with implications for understanding the regulatory and evolutionary dynamics of NLRs in the immune responses of plants.",
author = "Parker, {Matthew T.} and Katarzyna Knop and Vasiliki Zacharaki and Anna Sherwood and Daniel Tom{\'e} and Xuhong Yu and Pascal Martin and Jim Beynon and Scott Michaels and Geoffrey Barton and Simpson, {Gordon G.}",
year = "2021",
doi = "10.7554/ELIFE.65537",
language = "English",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications Ltd.",

}

RIS

TY - JOUR

T1 - Widespread premature transcription termination of Arabidopsis thaliana NLR genes by the spen protein FPA

AU - Parker, Matthew T.

AU - Knop, Katarzyna

AU - Zacharaki, Vasiliki

AU - Sherwood, Anna

AU - Tomé, Daniel

AU - Yu, Xuhong

AU - Martin, Pascal

AU - Beynon, Jim

AU - Michaels, Scott

AU - Barton, Geoffrey

AU - Simpson, Gordon G.

PY - 2021

Y1 - 2021

N2 - Genes involved in disease resistance are some of the fastest evolving and most diverse components of genomes. Large numbers of nucleotide-binding, leucine-rich repeat (NLR) genes are found in plant genomes and are required for disease resistance. However, NLRs can trigger autoimmunity, disrupt beneficial microbiota or reduce fitness. It is therefore crucial to understand how NLRs are controlled. Here, we show that the RNA-binding protein FPA mediates widespread premature cleavage and polyadenylation of NLR transcripts, thereby controlling their functional expression and impacting immunity. Using long-read Nanopore direct RNA sequencing, we resolved the complexity of NLR transcript processing and gene annotation. Our results uncover a co-transcriptional layer of NLR control with implications for understanding the regulatory and evolutionary dynamics of NLRs in the immune responses of plants.

AB - Genes involved in disease resistance are some of the fastest evolving and most diverse components of genomes. Large numbers of nucleotide-binding, leucine-rich repeat (NLR) genes are found in plant genomes and are required for disease resistance. However, NLRs can trigger autoimmunity, disrupt beneficial microbiota or reduce fitness. It is therefore crucial to understand how NLRs are controlled. Here, we show that the RNA-binding protein FPA mediates widespread premature cleavage and polyadenylation of NLR transcripts, thereby controlling their functional expression and impacting immunity. Using long-read Nanopore direct RNA sequencing, we resolved the complexity of NLR transcript processing and gene annotation. Our results uncover a co-transcriptional layer of NLR control with implications for understanding the regulatory and evolutionary dynamics of NLRs in the immune responses of plants.

U2 - 10.7554/ELIFE.65537

DO - 10.7554/ELIFE.65537

M3 - Journal article

JO - eLife

JF - eLife

SN - 2050-084X

ER -

ID: 368628105