7-Deaza purine bases offer a higher ion stability in the analysis of dna by matrix-assisted laser desorption/ionization mass spectrometry
Research output: Contribution to journal › Journal article › Research › peer-review
Oligodeoxynucleotides which contain 7-deaza analogues of the normal purine nucleotides have been synthesized both enzymatically and chemically. When subjected to matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis, the modified samples offer both higher stability and increased sensitivity compared to otherwise identical unmodified Oligodeoxynucleotides. In view of these observations, models for the fragmentation of oligodeoxynucleotides in MALDI-MS with positive ion detection mode are presented. Additionally, the potential use of 7-deaza purine nucleotides in the MALDI-MS analysis of DNA sequencing reactions is discussed.
|Journal||Rapid Communications in Mass Spectrometry|
|Pages (from-to)||525 - 531|
|Publication status||Published - 1995|