7-Deaza purine bases offer a higher ion stability in the analysis of dna by matrix-assisted laser desorption/ionization mass spectrometry

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Oligodeoxynucleotides which contain 7-deaza analogues of the normal purine nucleotides have been synthesized both enzymatically and chemically. When subjected to matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis, the modified samples offer both higher stability and increased sensitivity compared to otherwise identical unmodified Oligodeoxynucleotides. In view of these observations, models for the fragmentation of oligodeoxynucleotides in MALDI-MS with positive ion detection mode are presented. Additionally, the potential use of 7-deaza purine nucleotides in the MALDI-MS analysis of DNA sequencing reactions is discussed.
Original languageEnglish
JournalRapid Communications in Mass Spectrometry
Issue number6
Pages (from-to)525 - 531
Publication statusPublished - 1995
Externally publishedYes

ID: 11231924