ALG-2 knockdown in HeLa cells results in G2/M cell cycle phase accumulation and cell death

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Standard

ALG-2 knockdown in HeLa cells results in G2/M cell cycle phase accumulation and cell death. / Høj, Berit Rahbek; la Cour, Peter Jonas Marstrand; Mollerup, Jens; Berchtold, Martin Werner.

In: Biochemical and Biophysical Research Communications, Vol. 378, No. 1, 2009, p. 145-8.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Høj, BR, la Cour, PJM, Mollerup, J & Berchtold, MW 2009, 'ALG-2 knockdown in HeLa cells results in G2/M cell cycle phase accumulation and cell death', Biochemical and Biophysical Research Communications, vol. 378, no. 1, pp. 145-8. https://doi.org/10.1016/j.bbrc.2008.11.021

APA

Høj, B. R., la Cour, P. J. M., Mollerup, J., & Berchtold, M. W. (2009). ALG-2 knockdown in HeLa cells results in G2/M cell cycle phase accumulation and cell death. Biochemical and Biophysical Research Communications, 378(1), 145-8. https://doi.org/10.1016/j.bbrc.2008.11.021

Vancouver

Høj BR, la Cour PJM, Mollerup J, Berchtold MW. ALG-2 knockdown in HeLa cells results in G2/M cell cycle phase accumulation and cell death. Biochemical and Biophysical Research Communications. 2009;378(1):145-8. https://doi.org/10.1016/j.bbrc.2008.11.021

Author

Høj, Berit Rahbek ; la Cour, Peter Jonas Marstrand ; Mollerup, Jens ; Berchtold, Martin Werner. / ALG-2 knockdown in HeLa cells results in G2/M cell cycle phase accumulation and cell death. In: Biochemical and Biophysical Research Communications. 2009 ; Vol. 378, No. 1. pp. 145-8.

Bibtex

@article{3ef3c910cb7111dd9473000ea68e967b,
title = "ALG-2 knockdown in HeLa cells results in G2/M cell cycle phase accumulation and cell death",
abstract = "ALG-2 (apoptosis-linked gene-2 encoded protein) has been shown to be upregulated in a variety of human tumors questioning its previously assumed pro-apoptotic function. The aim of the present study was to obtain insights into the role of ALG-2 in human cancer cells. We show that ALG-2 downregulation induces accumulation of HeLa cells in the G2/M cell cycle phase and increases the amount of early apoptotic and dead cells. Caspase inhibition by the pan-caspase inhibitor zVAD-fmk attenuated the increase in the amount of dead cells following ALG-2 downregulation. Thus, our results indicate that ALG-2 has an anti-apoptotic function in HeLa cells by facilitating the passage through checkpoints in the G2/M cell cycle phase.",
author = "H{\o}j, {Berit Rahbek} and {la Cour}, {Peter Jonas Marstrand} and Jens Mollerup and Berchtold, {Martin Werner}",
note = "KEYWORDS: Calcium-binding proteins; Apoptosis-linked gene-2 (ALG-2); siRNA; Apoptosis; Cell cycle; Caspase",
year = "2009",
doi = "10.1016/j.bbrc.2008.11.021",
language = "English",
volume = "378",
pages = "145--8",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - ALG-2 knockdown in HeLa cells results in G2/M cell cycle phase accumulation and cell death

AU - Høj, Berit Rahbek

AU - la Cour, Peter Jonas Marstrand

AU - Mollerup, Jens

AU - Berchtold, Martin Werner

N1 - KEYWORDS: Calcium-binding proteins; Apoptosis-linked gene-2 (ALG-2); siRNA; Apoptosis; Cell cycle; Caspase

PY - 2009

Y1 - 2009

N2 - ALG-2 (apoptosis-linked gene-2 encoded protein) has been shown to be upregulated in a variety of human tumors questioning its previously assumed pro-apoptotic function. The aim of the present study was to obtain insights into the role of ALG-2 in human cancer cells. We show that ALG-2 downregulation induces accumulation of HeLa cells in the G2/M cell cycle phase and increases the amount of early apoptotic and dead cells. Caspase inhibition by the pan-caspase inhibitor zVAD-fmk attenuated the increase in the amount of dead cells following ALG-2 downregulation. Thus, our results indicate that ALG-2 has an anti-apoptotic function in HeLa cells by facilitating the passage through checkpoints in the G2/M cell cycle phase.

AB - ALG-2 (apoptosis-linked gene-2 encoded protein) has been shown to be upregulated in a variety of human tumors questioning its previously assumed pro-apoptotic function. The aim of the present study was to obtain insights into the role of ALG-2 in human cancer cells. We show that ALG-2 downregulation induces accumulation of HeLa cells in the G2/M cell cycle phase and increases the amount of early apoptotic and dead cells. Caspase inhibition by the pan-caspase inhibitor zVAD-fmk attenuated the increase in the amount of dead cells following ALG-2 downregulation. Thus, our results indicate that ALG-2 has an anti-apoptotic function in HeLa cells by facilitating the passage through checkpoints in the G2/M cell cycle phase.

U2 - 10.1016/j.bbrc.2008.11.021

DO - 10.1016/j.bbrc.2008.11.021

M3 - Journal article

C2 - 19013425

VL - 378

SP - 145

EP - 148

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -

ID: 9177448