ALG-2 knockdown in HeLa cells results in G2/M cell cycle phase accumulation and cell death
Research output: Contribution to journal › Journal article › Research › peer-review
ALG-2 (apoptosis-linked gene-2 encoded protein) has been shown to be upregulated in a variety of human tumors questioning its previously assumed pro-apoptotic function. The aim of the present study was to obtain insights into the role of ALG-2 in human cancer cells. We show that ALG-2 downregulation induces accumulation of HeLa cells in the G2/M cell cycle phase and increases the amount of early apoptotic and dead cells. Caspase inhibition by the pan-caspase inhibitor zVAD-fmk attenuated the increase in the amount of dead cells following ALG-2 downregulation. Thus, our results indicate that ALG-2 has an anti-apoptotic function in HeLa cells by facilitating the passage through checkpoints in the G2/M cell cycle phase.
Original language | English |
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Journal | Biochemical and Biophysical Research Communications |
Volume | 378 |
Issue number | 1 |
Pages (from-to) | 145-8 |
Number of pages | 3 |
ISSN | 0006-291X |
DOIs | |
Publication status | Published - 2009 |
Bibliographical note
KEYWORDS: Calcium-binding proteins; Apoptosis-linked gene-2 (ALG-2); siRNA; Apoptosis; Cell cycle; Caspase
ID: 9177448