Chitin and stress induced protein kinase activation

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Chitin and stress induced protein kinase activation. / Kenchappa, Chandra Shekar; Azevedo da Silva, Raquel; Bressendorff, Simon; Stanimirovic, Sabrina; Olsen, Jakob Valdbjørn; Petersen, Morten; Mundy, John.

Plant pattern recognition receptors: methods and protocols. ed. / Libo Shan; Ping He. Springer, 2017. p. 185-194 (Methods in Molecular Biology, Vol. 1578).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Kenchappa, CS, Azevedo da Silva, R, Bressendorff, S, Stanimirovic, S, Olsen, JV, Petersen, M & Mundy, J 2017, Chitin and stress induced protein kinase activation. in L Shan & P He (eds), Plant pattern recognition receptors: methods and protocols. Springer, Methods in Molecular Biology, vol. 1578, pp. 185-194. https://doi.org/10.1007/978-1-4939-6859-6_15

APA

Kenchappa, C. S., Azevedo da Silva, R., Bressendorff, S., Stanimirovic, S., Olsen, J. V., Petersen, M., & Mundy, J. (2017). Chitin and stress induced protein kinase activation. In L. Shan, & P. He (Eds.), Plant pattern recognition receptors: methods and protocols (pp. 185-194). Springer. Methods in Molecular Biology Vol. 1578 https://doi.org/10.1007/978-1-4939-6859-6_15

Vancouver

Kenchappa CS, Azevedo da Silva R, Bressendorff S, Stanimirovic S, Olsen JV, Petersen M et al. Chitin and stress induced protein kinase activation. In Shan L, He P, editors, Plant pattern recognition receptors: methods and protocols. Springer. 2017. p. 185-194. (Methods in Molecular Biology, Vol. 1578). https://doi.org/10.1007/978-1-4939-6859-6_15

Author

Kenchappa, Chandra Shekar ; Azevedo da Silva, Raquel ; Bressendorff, Simon ; Stanimirovic, Sabrina ; Olsen, Jakob Valdbjørn ; Petersen, Morten ; Mundy, John. / Chitin and stress induced protein kinase activation. Plant pattern recognition receptors: methods and protocols. editor / Libo Shan ; Ping He. Springer, 2017. pp. 185-194 (Methods in Molecular Biology, Vol. 1578).

Bibtex

@inbook{87466919347b4decb6b9f175f474df86,
title = "Chitin and stress induced protein kinase activation",
abstract = "The assays described here are pertinent to protein kinase studies in any plant. They include an immunoblot phosphorylation/activation assay and an in-gel activity assay for MAP kinases (MPKs) using the general protein kinase substrate myelin basic protein. They also include a novel in-gel peptide substrate assay for Snf1-related kinase family 2 members (SnRK2s). This kinase family-specific assay overcomes some limitations of in-gel assays and permits the identification of different types of kinase activities in total protein extracts.",
keywords = "Acryloylated peptide substrates, In-gel assays, Myelin basic protein",
author = "Kenchappa, {Chandra Shekar} and {Azevedo da Silva}, Raquel and Simon Bressendorff and Sabrina Stanimirovic and Olsen, {Jakob Valdbj{\o}rn} and Morten Petersen and John Mundy",
year = "2017",
doi = "10.1007/978-1-4939-6859-6_15",
language = "English",
isbn = "978-1-4939-6858-9",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "185--194",
editor = "Libo Shan and Ping He",
booktitle = "Plant pattern recognition receptors",
address = "Switzerland",

}

RIS

TY - CHAP

T1 - Chitin and stress induced protein kinase activation

AU - Kenchappa, Chandra Shekar

AU - Azevedo da Silva, Raquel

AU - Bressendorff, Simon

AU - Stanimirovic, Sabrina

AU - Olsen, Jakob Valdbjørn

AU - Petersen, Morten

AU - Mundy, John

PY - 2017

Y1 - 2017

N2 - The assays described here are pertinent to protein kinase studies in any plant. They include an immunoblot phosphorylation/activation assay and an in-gel activity assay for MAP kinases (MPKs) using the general protein kinase substrate myelin basic protein. They also include a novel in-gel peptide substrate assay for Snf1-related kinase family 2 members (SnRK2s). This kinase family-specific assay overcomes some limitations of in-gel assays and permits the identification of different types of kinase activities in total protein extracts.

AB - The assays described here are pertinent to protein kinase studies in any plant. They include an immunoblot phosphorylation/activation assay and an in-gel activity assay for MAP kinases (MPKs) using the general protein kinase substrate myelin basic protein. They also include a novel in-gel peptide substrate assay for Snf1-related kinase family 2 members (SnRK2s). This kinase family-specific assay overcomes some limitations of in-gel assays and permits the identification of different types of kinase activities in total protein extracts.

KW - Acryloylated peptide substrates

KW - In-gel assays

KW - Myelin basic protein

UR - http://www.scopus.com/inward/record.url?scp=85013647028&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-6859-6_15

DO - 10.1007/978-1-4939-6859-6_15

M3 - Book chapter

C2 - 28220425

AN - SCOPUS:85013647028

SN - 978-1-4939-6858-9

T3 - Methods in Molecular Biology

SP - 185

EP - 194

BT - Plant pattern recognition receptors

A2 - Shan, Libo

A2 - He, Ping

PB - Springer

ER -

ID: 181454851