Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides. / Nordhoff, E; Kirpekar, F; Karas, M; Cramer, R; Hahner, S; Hillenkamp, F; Kristiansen, K; Roepstroff, P; Lezius, A.

In: Nucleic Acids Research, Vol. 22, No. 13, 1994, p. 2460-5.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Nordhoff, E, Kirpekar, F, Karas, M, Cramer, R, Hahner, S, Hillenkamp, F, Kristiansen, K, Roepstroff, P & Lezius, A 1994, 'Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides', Nucleic Acids Research, vol. 22, no. 13, pp. 2460-5. <http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=308196>

APA

Nordhoff, E., Kirpekar, F., Karas, M., Cramer, R., Hahner, S., Hillenkamp, F., Kristiansen, K., Roepstroff, P., & Lezius, A. (1994). Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides. Nucleic Acids Research, 22(13), 2460-5. http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=308196

Vancouver

Nordhoff E, Kirpekar F, Karas M, Cramer R, Hahner S, Hillenkamp F et al. Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides. Nucleic Acids Research. 1994;22(13):2460-5.

Author

Nordhoff, E ; Kirpekar, F ; Karas, M ; Cramer, R ; Hahner, S ; Hillenkamp, F ; Kristiansen, K ; Roepstroff, P ; Lezius, A. / Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides. In: Nucleic Acids Research. 1994 ; Vol. 22, No. 13. pp. 2460-5.

Bibtex

@article{febbdae00f0911de8478000ea68e967b,
title = "Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides",
abstract = "UV-matrix assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS) with 3-hydroxypicolinic acid as matrix and IR-MALDI-MS with succinic acid as matrix have proved their feasibility for highly accurate and sensitive mass determination of nucleic acids (DNA and RNA). In this work, a detailed comparison of these two MALDI-methods and between positive- and negative ion mass spectra for the analysis of oligodeoxynucleotides is undertaken. Mass spectra of DNA sequences with up to 40 nucleotides are shown. Both linear and reflectron time-of-flight mass analyzers were used within this study and are compared for their potential in the MALDI analysis of oligodeoxynucleotides. The role of molecule-ion fragmentation is also discussed.",
author = "E Nordhoff and F Kirpekar and M Karas and R Cramer and S Hahner and F Hillenkamp and K Kristiansen and P Roepstroff and A Lezius",
note = "Keywords: Base Sequence; Lasers; Mass Spectrometry; Molecular Sequence Data; Oligodeoxyribonucleotides; Picolinic Acids; Spectrophotometry, Infrared; Spectrophotometry, Ultraviolet; Succinates; Succinic Acid",
year = "1994",
language = "English",
volume = "22",
pages = "2460--5",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "13",

}

RIS

TY - JOUR

T1 - Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides

AU - Nordhoff, E

AU - Kirpekar, F

AU - Karas, M

AU - Cramer, R

AU - Hahner, S

AU - Hillenkamp, F

AU - Kristiansen, K

AU - Roepstroff, P

AU - Lezius, A

N1 - Keywords: Base Sequence; Lasers; Mass Spectrometry; Molecular Sequence Data; Oligodeoxyribonucleotides; Picolinic Acids; Spectrophotometry, Infrared; Spectrophotometry, Ultraviolet; Succinates; Succinic Acid

PY - 1994

Y1 - 1994

N2 - UV-matrix assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS) with 3-hydroxypicolinic acid as matrix and IR-MALDI-MS with succinic acid as matrix have proved their feasibility for highly accurate and sensitive mass determination of nucleic acids (DNA and RNA). In this work, a detailed comparison of these two MALDI-methods and between positive- and negative ion mass spectra for the analysis of oligodeoxynucleotides is undertaken. Mass spectra of DNA sequences with up to 40 nucleotides are shown. Both linear and reflectron time-of-flight mass analyzers were used within this study and are compared for their potential in the MALDI analysis of oligodeoxynucleotides. The role of molecule-ion fragmentation is also discussed.

AB - UV-matrix assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS) with 3-hydroxypicolinic acid as matrix and IR-MALDI-MS with succinic acid as matrix have proved their feasibility for highly accurate and sensitive mass determination of nucleic acids (DNA and RNA). In this work, a detailed comparison of these two MALDI-methods and between positive- and negative ion mass spectra for the analysis of oligodeoxynucleotides is undertaken. Mass spectra of DNA sequences with up to 40 nucleotides are shown. Both linear and reflectron time-of-flight mass analyzers were used within this study and are compared for their potential in the MALDI analysis of oligodeoxynucleotides. The role of molecule-ion fragmentation is also discussed.

M3 - Journal article

C2 - 8041606

VL - 22

SP - 2460

EP - 2465

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 13

ER -

ID: 11231552