Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides
Research output: Contribution to journal › Journal article › Research › peer-review
UV-matrix assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS) with 3-hydroxypicolinic acid as matrix and IR-MALDI-MS with succinic acid as matrix have proved their feasibility for highly accurate and sensitive mass determination of nucleic acids (DNA and RNA). In this work, a detailed comparison of these two MALDI-methods and between positive- and negative ion mass spectra for the analysis of oligodeoxynucleotides is undertaken. Mass spectra of DNA sequences with up to 40 nucleotides are shown. Both linear and reflectron time-of-flight mass analyzers were used within this study and are compared for their potential in the MALDI analysis of oligodeoxynucleotides. The role of molecule-ion fragmentation is also discussed.
|Journal||Nucleic Acids Research|
|Number of pages||5|
|Publication status||Published - 1994|
Keywords: Base Sequence; Lasers; Mass Spectrometry; Molecular Sequence Data; Oligodeoxyribonucleotides; Picolinic Acids; Spectrophotometry, Infrared; Spectrophotometry, Ultraviolet; Succinates; Succinic Acid