Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides

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UV-matrix assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS) with 3-hydroxypicolinic acid as matrix and IR-MALDI-MS with succinic acid as matrix have proved their feasibility for highly accurate and sensitive mass determination of nucleic acids (DNA and RNA). In this work, a detailed comparison of these two MALDI-methods and between positive- and negative ion mass spectra for the analysis of oligodeoxynucleotides is undertaken. Mass spectra of DNA sequences with up to 40 nucleotides are shown. Both linear and reflectron time-of-flight mass analyzers were used within this study and are compared for their potential in the MALDI analysis of oligodeoxynucleotides. The role of molecule-ion fragmentation is also discussed.
Original languageEnglish
JournalNucleic Acids Research
Issue number13
Pages (from-to)2460-5
Number of pages5
Publication statusPublished - 1994
Externally publishedYes

Bibliographical note

Keywords: Base Sequence; Lasers; Mass Spectrometry; Molecular Sequence Data; Oligodeoxyribonucleotides; Picolinic Acids; Spectrophotometry, Infrared; Spectrophotometry, Ultraviolet; Succinates; Succinic Acid

ID: 11231552