Improved quantification of stress proteins by western blotting

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Improved quantification of stress proteins by western blotting. / Lundebye, Anne-Katrine; R. Vedel, Grace; Christensen, Anne M. Krogsdam; Kristiansen, Karsten; Hunter, Douglas; Depledge, Michael H.

In: Analytica Chimica Acta, Vol. 311, No. 1, 1995, p. 109-114.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Lundebye, A-K, R. Vedel, G, Christensen, AMK, Kristiansen, K, Hunter, D & Depledge, MH 1995, 'Improved quantification of stress proteins by western blotting', Analytica Chimica Acta, vol. 311, no. 1, pp. 109-114. https://doi.org/10.1016/0003-2670(95)00162-S

APA

Lundebye, A-K., R. Vedel, G., Christensen, A. M. K., Kristiansen, K., Hunter, D., & Depledge, M. H. (1995). Improved quantification of stress proteins by western blotting. Analytica Chimica Acta, 311(1), 109-114. https://doi.org/10.1016/0003-2670(95)00162-S

Vancouver

Lundebye A-K, R. Vedel G, Christensen AMK, Kristiansen K, Hunter D, Depledge MH. Improved quantification of stress proteins by western blotting. Analytica Chimica Acta. 1995;311(1):109-114. https://doi.org/10.1016/0003-2670(95)00162-S

Author

Lundebye, Anne-Katrine ; R. Vedel, Grace ; Christensen, Anne M. Krogsdam ; Kristiansen, Karsten ; Hunter, Douglas ; Depledge, Michael H. / Improved quantification of stress proteins by western blotting. In: Analytica Chimica Acta. 1995 ; Vol. 311, No. 1. pp. 109-114.

Bibtex

@article{b47dfd700f1111de8478000ea68e967b,
title = "Improved quantification of stress proteins by western blotting",
abstract = "An improved method for the semi-quantification of stress protein biomarkers by western blotting is described. The construction of a calibration curve on each gel (using a standard) to calculate relative stress protein levels improved reproducibility of results both within and among blots. Variability in relative stress protein levels of replicate samples run on separate gels was reduced from ca. 24% to 13% when related to a calibration curve rather than being based on direct comparison of absorbance units. Within blot variability was reduced from 5.2-11.7% to 3.4-6.8% by calibrating stress-70 levels. The potential significance of this methodological development is discussed with regard to the literature.",
author = "Anne-Katrine Lundebye and {R. Vedel}, Grace and Christensen, {Anne M. Krogsdam} and Karsten Kristiansen and Douglas Hunter and Depledge, {Michael H.}",
note = "Western blotting; Stress proteins; Proteins; Carcinus maenas; Mytilus edulis",
year = "1995",
doi = "10.1016/0003-2670(95)00162-S",
language = "English",
volume = "311",
pages = "109--114",
journal = "Analytica Chimica Acta",
issn = "0003-2670",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Improved quantification of stress proteins by western blotting

AU - Lundebye, Anne-Katrine

AU - R. Vedel, Grace

AU - Christensen, Anne M. Krogsdam

AU - Kristiansen, Karsten

AU - Hunter, Douglas

AU - Depledge, Michael H.

N1 - Western blotting; Stress proteins; Proteins; Carcinus maenas; Mytilus edulis

PY - 1995

Y1 - 1995

N2 - An improved method for the semi-quantification of stress protein biomarkers by western blotting is described. The construction of a calibration curve on each gel (using a standard) to calculate relative stress protein levels improved reproducibility of results both within and among blots. Variability in relative stress protein levels of replicate samples run on separate gels was reduced from ca. 24% to 13% when related to a calibration curve rather than being based on direct comparison of absorbance units. Within blot variability was reduced from 5.2-11.7% to 3.4-6.8% by calibrating stress-70 levels. The potential significance of this methodological development is discussed with regard to the literature.

AB - An improved method for the semi-quantification of stress protein biomarkers by western blotting is described. The construction of a calibration curve on each gel (using a standard) to calculate relative stress protein levels improved reproducibility of results both within and among blots. Variability in relative stress protein levels of replicate samples run on separate gels was reduced from ca. 24% to 13% when related to a calibration curve rather than being based on direct comparison of absorbance units. Within blot variability was reduced from 5.2-11.7% to 3.4-6.8% by calibrating stress-70 levels. The potential significance of this methodological development is discussed with regard to the literature.

U2 - 10.1016/0003-2670(95)00162-S

DO - 10.1016/0003-2670(95)00162-S

M3 - Journal article

VL - 311

SP - 109

EP - 114

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

SN - 0003-2670

IS - 1

ER -

ID: 11231984