Improved quantification of stress proteins by western blotting

Research output: Contribution to journalJournal articleResearchpeer-review

  • Anne-Katrine Lundebye
  • Grace R. Vedel
  • Anne M. Krogsdam Christensen
  • Kristiansen, Karsten
  • Douglas Hunter
  • Michael H. Depledge
An improved method for the semi-quantification of stress protein biomarkers by western blotting is described. The construction of a calibration curve on each gel (using a standard) to calculate relative stress protein levels improved reproducibility of results both within and among blots. Variability in relative stress protein levels of replicate samples run on separate gels was reduced from ca. 24% to 13% when related to a calibration curve rather than being based on direct comparison of absorbance units. Within blot variability was reduced from 5.2-11.7% to 3.4-6.8% by calibrating stress-70 levels. The potential significance of this methodological development is discussed with regard to the literature.
Original languageEnglish
JournalAnalytica Chimica Acta
Issue number1
Pages (from-to)109-114
Publication statusPublished - 1995
Externally publishedYes

Bibliographical note

Western blotting; Stress proteins; Proteins; Carcinus maenas; Mytilus edulis

ID: 11231984