Induction of acyl-CoA-binding protein and its mRNA in 3T3-L1 cells by insulin during preadipocyte-to-adipocyte differentiation

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Induction of acyl-CoA-binding protein and its mRNA in 3T3-L1 cells by insulin during preadipocyte-to-adipocyte differentiation. / Hansen, H O; Andreasen, P H; Mandrup, S; Kristiansen, K; Knudsen, J.

In: Biochemical Journal, Vol. 277 ( Pt 2), 1991, p. 341-4.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hansen, HO, Andreasen, PH, Mandrup, S, Kristiansen, K & Knudsen, J 1991, 'Induction of acyl-CoA-binding protein and its mRNA in 3T3-L1 cells by insulin during preadipocyte-to-adipocyte differentiation', Biochemical Journal, vol. 277 ( Pt 2), pp. 341-4. <http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1151239>

APA

Hansen, H. O., Andreasen, P. H., Mandrup, S., Kristiansen, K., & Knudsen, J. (1991). Induction of acyl-CoA-binding protein and its mRNA in 3T3-L1 cells by insulin during preadipocyte-to-adipocyte differentiation. Biochemical Journal, 277 ( Pt 2), 341-4. http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1151239

Vancouver

Hansen HO, Andreasen PH, Mandrup S, Kristiansen K, Knudsen J. Induction of acyl-CoA-binding protein and its mRNA in 3T3-L1 cells by insulin during preadipocyte-to-adipocyte differentiation. Biochemical Journal. 1991;277 ( Pt 2):341-4.

Author

Hansen, H O ; Andreasen, P H ; Mandrup, S ; Kristiansen, K ; Knudsen, J. / Induction of acyl-CoA-binding protein and its mRNA in 3T3-L1 cells by insulin during preadipocyte-to-adipocyte differentiation. In: Biochemical Journal. 1991 ; Vol. 277 ( Pt 2). pp. 341-4.

Bibtex

@article{c3734c500fc911de8478000ea68e967b,
title = "Induction of acyl-CoA-binding protein and its mRNA in 3T3-L1 cells by insulin during preadipocyte-to-adipocyte differentiation",
abstract = "The induction of acyl-CoA-binding protein (ACBP) and ACBP mRNA was investigated in 3T3-L1 cells during growth and insulin-induced differentiation. The level of ACBP relative to both total soluble protein and DNA increased during insulin-stimulated conversion of 3T3-L1 cells from preadipocytes into fully developed adipocytes. So did the total rate of lipogenesis, as measured by incorporation of [1-14C]acetate. A similar increase in ACBP mRNA relative to total RNA was observed. These results therefore suggest that ACBP plays a specific role in the lipogenic process. However, this role might be indirect, as the increase in lipogenesis preceded the increase in ACBP. The significance of this finding is discussed.",
author = "Hansen, {H O} and Andreasen, {P H} and S Mandrup and K Kristiansen and J Knudsen",
note = "Keywords: Acetates; Adipose Tissue; Animals; Carrier Proteins; Cell Differentiation; Cell Line; Cloning, Molecular; Dexamethasone; Diazepam Binding Inhibitor; Gene Library; Kinetics; Lipids; Liver; Mice; RNA, Messenger; Rats; Rats, Inbred Strains; Time Factors",
year = "1991",
language = "English",
volume = "277 ( Pt 2)",
pages = "341--4",
journal = "Biochemical Journal",
issn = "0264-6021",
publisher = "Portland Press Ltd.",

}

RIS

TY - JOUR

T1 - Induction of acyl-CoA-binding protein and its mRNA in 3T3-L1 cells by insulin during preadipocyte-to-adipocyte differentiation

AU - Hansen, H O

AU - Andreasen, P H

AU - Mandrup, S

AU - Kristiansen, K

AU - Knudsen, J

N1 - Keywords: Acetates; Adipose Tissue; Animals; Carrier Proteins; Cell Differentiation; Cell Line; Cloning, Molecular; Dexamethasone; Diazepam Binding Inhibitor; Gene Library; Kinetics; Lipids; Liver; Mice; RNA, Messenger; Rats; Rats, Inbred Strains; Time Factors

PY - 1991

Y1 - 1991

N2 - The induction of acyl-CoA-binding protein (ACBP) and ACBP mRNA was investigated in 3T3-L1 cells during growth and insulin-induced differentiation. The level of ACBP relative to both total soluble protein and DNA increased during insulin-stimulated conversion of 3T3-L1 cells from preadipocytes into fully developed adipocytes. So did the total rate of lipogenesis, as measured by incorporation of [1-14C]acetate. A similar increase in ACBP mRNA relative to total RNA was observed. These results therefore suggest that ACBP plays a specific role in the lipogenic process. However, this role might be indirect, as the increase in lipogenesis preceded the increase in ACBP. The significance of this finding is discussed.

AB - The induction of acyl-CoA-binding protein (ACBP) and ACBP mRNA was investigated in 3T3-L1 cells during growth and insulin-induced differentiation. The level of ACBP relative to both total soluble protein and DNA increased during insulin-stimulated conversion of 3T3-L1 cells from preadipocytes into fully developed adipocytes. So did the total rate of lipogenesis, as measured by incorporation of [1-14C]acetate. A similar increase in ACBP mRNA relative to total RNA was observed. These results therefore suggest that ACBP plays a specific role in the lipogenic process. However, this role might be indirect, as the increase in lipogenesis preceded the increase in ACBP. The significance of this finding is discussed.

M3 - Journal article

C2 - 1859362

VL - 277 ( Pt 2)

SP - 341

EP - 344

JO - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

ER -

ID: 11255484