Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa
Research output: Contribution to journal › Journal article › Research › peer-review
Enzymatically synthesized RNA samples (in vitro transcripts) were analysed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Spectra of RNA up to 150 kDA (461 nucleotides) are shown. Polymerase generated sample heterogeneity and its contribution to mass resolution are discussed. A time course exonuclease digest of a 55 nt in vitro transcript was analyzed to investigate the performance of MALDI-MS on complex mixtures. Based on these data, the analysis by MALDI-MS of DNA sequencing reactions, produced by the action of an RNA polymerase, is discussed.
| Original language | English |
|---|---|
| Journal | Nucleic Acids Research |
| Volume | 22 |
| Issue number | 19 |
| Pages (from-to) | 3866-70 |
| Number of pages | 4 |
| ISSN | 0305-1048 |
| Publication status | Published - 1994 |
| Externally published | Yes |
Bibliographical note
Keywords: Base Sequence; DNA-Directed RNA Polymerases; Lasers; Mass Spectrometry; Molecular Sequence Data; Molecular Weight; Phosphoric Diester Hydrolases; RNA; Sequence Analysis, RNA
ID: 11231720