The early noncoding region of human papillomavirus type 16 is regulated by cytoplasmic polyadenylation factors

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The early noncoding region of human papillomavirus type 16 is regulated by cytoplasmic polyadenylation factors. / Glahder, Jacob-Andreas Harald; Kristiansen, Karen; Durand, Marjorie; Vinther, Jeppe; Norrild, Bodil.

In: Virus Research, Vol. 149, No. 2, 2010, p. 217-223.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Glahder, J-AH, Kristiansen, K, Durand, M, Vinther, J & Norrild, B 2010, 'The early noncoding region of human papillomavirus type 16 is regulated by cytoplasmic polyadenylation factors', Virus Research, vol. 149, no. 2, pp. 217-223. https://doi.org/10.1016/j.virusres.2010.02.001

APA

Glahder, J-A. H., Kristiansen, K., Durand, M., Vinther, J., & Norrild, B. (2010). The early noncoding region of human papillomavirus type 16 is regulated by cytoplasmic polyadenylation factors. Virus Research, 149(2), 217-223. https://doi.org/10.1016/j.virusres.2010.02.001

Vancouver

Glahder J-AH, Kristiansen K, Durand M, Vinther J, Norrild B. The early noncoding region of human papillomavirus type 16 is regulated by cytoplasmic polyadenylation factors. Virus Research. 2010;149(2):217-223. https://doi.org/10.1016/j.virusres.2010.02.001

Author

Glahder, Jacob-Andreas Harald ; Kristiansen, Karen ; Durand, Marjorie ; Vinther, Jeppe ; Norrild, Bodil. / The early noncoding region of human papillomavirus type 16 is regulated by cytoplasmic polyadenylation factors. In: Virus Research. 2010 ; Vol. 149, No. 2. pp. 217-223.

Bibtex

@article{c28f67f038d211dfad7f000ea68e967b,

title = "The early noncoding region of human papillomavirus type 16 is regulated by cytoplasmic polyadenylation factors",

abstract = "All human papillomavirus type 16 (HPV-16) early mRNAs are polyadenylated at the poly(A) signal within the early 3' untranslated region (3'UTR). The 3'end of the early E5 open reading frame and the 3'UTR of HPV-16 is very AU-rich, with five regions similar to cytoplasmic polyadenylation elements (CPEs). We show here that a fragment of the early 3'end comprising four of the five CPE-like regions when inserted downstream of a reporter gene confers regulation of the gene expression. A key protein involved in cytoplasmic polyadenylation is CPEB. We show that the human CPEB1 can repress the activity of the reporter construct containing the HPV-16 early sequences. This repression can be counteracted by a human cytoplasmic poly(A) polymerase, hGLD-2 fused to CPEB1. The hGLD-2/CPEB1 fusion protein facilitates furthermore poly(A) elongation of early HPV transcripts.",

author = "Glahder, {Jacob-Andreas Harald} and Karen Kristiansen and Marjorie Durand and Jeppe Vinther and Bodil Norrild",

year = "2010",

doi = "10.1016/j.virusres.2010.02.001",

language = "English",

volume = "149",

pages = "217--223",

journal = "Virus Research",

issn = "0168-1702",

publisher = "Elsevier",

number = "2",

}

RIS

TY - JOUR

T1 - The early noncoding region of human papillomavirus type 16 is regulated by cytoplasmic polyadenylation factors

AU - Glahder, Jacob-Andreas Harald

AU - Kristiansen, Karen

AU - Durand, Marjorie

AU - Vinther, Jeppe

AU - Norrild, Bodil

PY - 2010

Y1 - 2010

N2 - All human papillomavirus type 16 (HPV-16) early mRNAs are polyadenylated at the poly(A) signal within the early 3' untranslated region (3'UTR). The 3'end of the early E5 open reading frame and the 3'UTR of HPV-16 is very AU-rich, with five regions similar to cytoplasmic polyadenylation elements (CPEs). We show here that a fragment of the early 3'end comprising four of the five CPE-like regions when inserted downstream of a reporter gene confers regulation of the gene expression. A key protein involved in cytoplasmic polyadenylation is CPEB. We show that the human CPEB1 can repress the activity of the reporter construct containing the HPV-16 early sequences. This repression can be counteracted by a human cytoplasmic poly(A) polymerase, hGLD-2 fused to CPEB1. The hGLD-2/CPEB1 fusion protein facilitates furthermore poly(A) elongation of early HPV transcripts.

AB - All human papillomavirus type 16 (HPV-16) early mRNAs are polyadenylated at the poly(A) signal within the early 3' untranslated region (3'UTR). The 3'end of the early E5 open reading frame and the 3'UTR of HPV-16 is very AU-rich, with five regions similar to cytoplasmic polyadenylation elements (CPEs). We show here that a fragment of the early 3'end comprising four of the five CPE-like regions when inserted downstream of a reporter gene confers regulation of the gene expression. A key protein involved in cytoplasmic polyadenylation is CPEB. We show that the human CPEB1 can repress the activity of the reporter construct containing the HPV-16 early sequences. This repression can be counteracted by a human cytoplasmic poly(A) polymerase, hGLD-2 fused to CPEB1. The hGLD-2/CPEB1 fusion protein facilitates furthermore poly(A) elongation of early HPV transcripts.

U2 - 10.1016/j.virusres.2010.02.001

DO - 10.1016/j.virusres.2010.02.001

M3 - Journal article

C2 - 20144904

VL - 149

SP - 217

EP - 223

JO - Virus Research

JF - Virus Research

SN - 0168-1702

IS - 2

ER -

ID: 18838439

Department of Biology