The early noncoding region of human papillomavirus type 16 is regulated by cytoplasmic polyadenylation factors
Research output: Contribution to journal › Journal article › Research › peer-review
All human papillomavirus type 16 (HPV-16) early mRNAs are polyadenylated at the poly(A) signal within the early 3' untranslated region (3'UTR). The 3'end of the early E5 open reading frame and the 3'UTR of HPV-16 is very AU-rich, with five regions similar to cytoplasmic polyadenylation elements (CPEs). We show here that a fragment of the early 3'end comprising four of the five CPE-like regions when inserted downstream of a reporter gene confers regulation of the gene expression. A key protein involved in cytoplasmic polyadenylation is CPEB. We show that the human CPEB1 can repress the activity of the reporter construct containing the HPV-16 early sequences. This repression can be counteracted by a human cytoplasmic poly(A) polymerase, hGLD-2 fused to CPEB1. The hGLD-2/CPEB1 fusion protein facilitates furthermore poly(A) elongation of early HPV transcripts.
Original language | English |
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Journal | Virus Research |
Volume | 149 |
Issue number | 2 |
Pages (from-to) | 217-223 |
Number of pages | 6 |
ISSN | 0168-1702 |
DOIs | |
Publication status | Published - 2010 |
ID: 18838439