The PEF family proteins sorcin and grancalcin interact in vivo and in vitro.

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Standard

The PEF family proteins sorcin and grancalcin interact in vivo and in vitro. / Hansen, Christian; Tarabykina, Svetlana; la Cour, Jonas Marstrand; Lollike, Karsten; Berchtold, Martin W.

In: FEBS Letters, Vol. 545, No. 2-3, 2003, p. 151-4.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hansen, C, Tarabykina, S, la Cour, JM, Lollike, K & Berchtold, MW 2003, 'The PEF family proteins sorcin and grancalcin interact in vivo and in vitro.', FEBS Letters, vol. 545, no. 2-3, pp. 151-4. https://doi.org/10.1016/S0014-5793(03)00518-0

APA

Hansen, C., Tarabykina, S., la Cour, J. M., Lollike, K., & Berchtold, M. W. (2003). The PEF family proteins sorcin and grancalcin interact in vivo and in vitro. FEBS Letters, 545(2-3), 151-4. https://doi.org/10.1016/S0014-5793(03)00518-0

Vancouver

Hansen C, Tarabykina S, la Cour JM, Lollike K, Berchtold MW. The PEF family proteins sorcin and grancalcin interact in vivo and in vitro. FEBS Letters. 2003;545(2-3):151-4. https://doi.org/10.1016/S0014-5793(03)00518-0

Author

Hansen, Christian ; Tarabykina, Svetlana ; la Cour, Jonas Marstrand ; Lollike, Karsten ; Berchtold, Martin W. / The PEF family proteins sorcin and grancalcin interact in vivo and in vitro. In: FEBS Letters. 2003 ; Vol. 545, No. 2-3. pp. 151-4.

Bibtex

@article{fbcb9b60f0f911dcbee902004c4f4f50,
title = "The PEF family proteins sorcin and grancalcin interact in vivo and in vitro.",
abstract = "The penta-EF hand (PEF) family of calcium binding proteins includes grancalcin, peflin, sorcin, calpain large and small subunits as well as ALG-2. Systematic testing of the heterodimerization abilities of the PEF proteins using the yeast two-hybrid and glutathione S-transferase pull-down assays revealed the new finding that grancalcin interacts strongly with sorcin. In addition, sorcin and grancalcin can be co-immunoprecipitated from lysates of human umbilical vein endothelial cells. Our results indicate that heterodimerization, in addition to differential interactions with target proteins, might be a way to regulate and fine tune processes mediated by calcium binding proteins of the penta-EF hand type.",
author = "Christian Hansen and Svetlana Tarabykina and {la Cour}, {Jonas Marstrand} and Karsten Lollike and Berchtold, {Martin W}",
note = "Keywords: Calcium-Binding Proteins; Cells, Cultured; Dimerization; EF Hand Motifs; Endothelium, Vascular; Glutathione Transferase; Humans; Precipitin Tests; Recombinant Fusion Proteins; Two-Hybrid System Techniques; Umbilical Veins",
year = "2003",
doi = "10.1016/S0014-5793(03)00518-0",
language = "English",
volume = "545",
pages = "151--4",
journal = "F E B S Letters",
issn = "0014-5793",
publisher = "JohnWiley & Sons Ltd",
number = "2-3",

}

RIS

TY - JOUR

T1 - The PEF family proteins sorcin and grancalcin interact in vivo and in vitro.

AU - Hansen, Christian

AU - Tarabykina, Svetlana

AU - la Cour, Jonas Marstrand

AU - Lollike, Karsten

AU - Berchtold, Martin W

N1 - Keywords: Calcium-Binding Proteins; Cells, Cultured; Dimerization; EF Hand Motifs; Endothelium, Vascular; Glutathione Transferase; Humans; Precipitin Tests; Recombinant Fusion Proteins; Two-Hybrid System Techniques; Umbilical Veins

PY - 2003

Y1 - 2003

N2 - The penta-EF hand (PEF) family of calcium binding proteins includes grancalcin, peflin, sorcin, calpain large and small subunits as well as ALG-2. Systematic testing of the heterodimerization abilities of the PEF proteins using the yeast two-hybrid and glutathione S-transferase pull-down assays revealed the new finding that grancalcin interacts strongly with sorcin. In addition, sorcin and grancalcin can be co-immunoprecipitated from lysates of human umbilical vein endothelial cells. Our results indicate that heterodimerization, in addition to differential interactions with target proteins, might be a way to regulate and fine tune processes mediated by calcium binding proteins of the penta-EF hand type.

AB - The penta-EF hand (PEF) family of calcium binding proteins includes grancalcin, peflin, sorcin, calpain large and small subunits as well as ALG-2. Systematic testing of the heterodimerization abilities of the PEF proteins using the yeast two-hybrid and glutathione S-transferase pull-down assays revealed the new finding that grancalcin interacts strongly with sorcin. In addition, sorcin and grancalcin can be co-immunoprecipitated from lysates of human umbilical vein endothelial cells. Our results indicate that heterodimerization, in addition to differential interactions with target proteins, might be a way to regulate and fine tune processes mediated by calcium binding proteins of the penta-EF hand type.

U2 - 10.1016/S0014-5793(03)00518-0

DO - 10.1016/S0014-5793(03)00518-0

M3 - Journal article

C2 - 12804766

VL - 545

SP - 151

EP - 154

JO - F E B S Letters

JF - F E B S Letters

SN - 0014-5793

IS - 2-3

ER -

ID: 3137575