The PEF family proteins sorcin and grancalcin interact in vivo and in vitro.
Research output: Contribution to journal › Journal article › Research › peer-review
The penta-EF hand (PEF) family of calcium binding proteins includes grancalcin, peflin, sorcin, calpain large and small subunits as well as ALG-2. Systematic testing of the heterodimerization abilities of the PEF proteins using the yeast two-hybrid and glutathione S-transferase pull-down assays revealed the new finding that grancalcin interacts strongly with sorcin. In addition, sorcin and grancalcin can be co-immunoprecipitated from lysates of human umbilical vein endothelial cells. Our results indicate that heterodimerization, in addition to differential interactions with target proteins, might be a way to regulate and fine tune processes mediated by calcium binding proteins of the penta-EF hand type.
Original language | English |
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Journal | FEBS Letters |
Volume | 545 |
Issue number | 2-3 |
Pages (from-to) | 151-4 |
Number of pages | 3 |
ISSN | 0014-5793 |
DOIs | |
Publication status | Published - 2003 |
Bibliographical note
Keywords: Calcium-Binding Proteins; Cells, Cultured; Dimerization; EF Hand Motifs; Endothelium, Vascular; Glutathione Transferase; Humans; Precipitin Tests; Recombinant Fusion Proteins; Two-Hybrid System Techniques; Umbilical Veins
ID: 3137575