Branchial versus intestinal silver toxicity and uptake in the marine teleost Parophrys vetulus
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Branchial versus intestinal silver toxicity and uptake in the marine teleost Parophrys vetulus. / Grosell, Martin Hautopp; Wood, C. M.
I: Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology, Bind 171, Nr. 7, 2001, s. 585-594.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Branchial versus intestinal silver toxicity and uptake in the marine teleost Parophrys vetulus
AU - Grosell, Martin Hautopp
AU - Wood, C. M.
PY - 2001
Y1 - 2001
N2 - Exposure to elevated waterborne silver as AgNO3 (4.07 µM=448 µg l-1) in seawater resulted in osmoregulatory disturbance in the lemon sole (Parophrys vetulus). The main effects were increased plasma Na+ and Cl- concentrations which translated into increased plasma osmolality. Plasma Mg2+ levels were also slightly increased after 96 h exposure. Using radio-isotopic flux measurements, a 50% reduction in branchial unidirectional Na+ extrusion was observed after 48 h silver exposure. By applying an intestinal perfusion approach, we were able to separate and thus quantify the intestinal contribution to the observed silver-induced physiological disturbance and internal silver accumulation. This analysis revealed that the intestinal contribution to silver-induced ionoregulatory toxicity was as high as 50-60%. In marked contrast, internal silver accumulation (in liver and kidney) was found to be derived exclusively from uptake across the gills. Drinking of silver-contaminated seawater resulted in substantial silver accumulation in the intestinal tissue (but apparently not silver uptake across the intestine), which probably explains the intestinal contribution to silver-induced physiological disturbance.
AB - Exposure to elevated waterborne silver as AgNO3 (4.07 µM=448 µg l-1) in seawater resulted in osmoregulatory disturbance in the lemon sole (Parophrys vetulus). The main effects were increased plasma Na+ and Cl- concentrations which translated into increased plasma osmolality. Plasma Mg2+ levels were also slightly increased after 96 h exposure. Using radio-isotopic flux measurements, a 50% reduction in branchial unidirectional Na+ extrusion was observed after 48 h silver exposure. By applying an intestinal perfusion approach, we were able to separate and thus quantify the intestinal contribution to the observed silver-induced physiological disturbance and internal silver accumulation. This analysis revealed that the intestinal contribution to silver-induced ionoregulatory toxicity was as high as 50-60%. In marked contrast, internal silver accumulation (in liver and kidney) was found to be derived exclusively from uptake across the gills. Drinking of silver-contaminated seawater resulted in substantial silver accumulation in the intestinal tissue (but apparently not silver uptake across the intestine), which probably explains the intestinal contribution to silver-induced physiological disturbance.
U2 - 10.1007/s003600100209
DO - 10.1007/s003600100209
M3 - Journal article
VL - 171
SP - 585
EP - 594
JO - Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
JF - Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
SN - 0174-1578
IS - 7
ER -
ID: 142844