Distribution of 3H within purine nucleotides of Ehrlich mouse ascites tumour cells after intraabdominal injection of myo-[2-3H]inositol

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Standard

Distribution of 3H within purine nucleotides of Ehrlich mouse ascites tumour cells after intraabdominal injection of myo-[2-3H]inositol. / Christensen, Søren; Klenow, H.; Overgaard-Hansen, Kay.

I: Journal of Labelled Compounds and Radiopharmaceuticals, Bind 43, Nr. 1, 2000, s. 91-96.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Christensen, S, Klenow, H & Overgaard-Hansen, K 2000, 'Distribution of 3H within purine nucleotides of Ehrlich mouse ascites tumour cells after intraabdominal injection of myo-[2-3H]inositol', Journal of Labelled Compounds and Radiopharmaceuticals, bind 43, nr. 1, s. 91-96. <http://www3.interscience.wiley.com/cgi-bin/fulltext/69501397/PDFSTART>

APA

Christensen, S., Klenow, H., & Overgaard-Hansen, K. (2000). Distribution of 3H within purine nucleotides of Ehrlich mouse ascites tumour cells after intraabdominal injection of myo-[2-3H]inositol. Journal of Labelled Compounds and Radiopharmaceuticals, 43(1), 91-96. http://www3.interscience.wiley.com/cgi-bin/fulltext/69501397/PDFSTART

Vancouver

Christensen S, Klenow H, Overgaard-Hansen K. Distribution of 3H within purine nucleotides of Ehrlich mouse ascites tumour cells after intraabdominal injection of myo-[2-3H]inositol. Journal of Labelled Compounds and Radiopharmaceuticals. 2000;43(1):91-96.

Author

Christensen, Søren ; Klenow, H. ; Overgaard-Hansen, Kay. / Distribution of 3H within purine nucleotides of Ehrlich mouse ascites tumour cells after intraabdominal injection of myo-[2-3H]inositol. I: Journal of Labelled Compounds and Radiopharmaceuticals. 2000 ; Bind 43, Nr. 1. s. 91-96.

Bibtex

@article{a7d8007074c211dbbee902004c4f4f50,
title = "Distribution of 3H within purine nucleotides of Ehrlich mouse ascites tumour cells after intraabdominal injection of myo-[2-3H]inositol",
abstract = "In Ehrlich mouse ascites tumour cells, exposed intra-abdominally to [2-3H]inositol, ATP and GTP presented enough aberrant 3H-label to cause potential interference in the chromatographic analysis of inositol phosphates involved in signal transduction. After acid extraction and charcoal adsorption/desorption the nucleotides were dephosphorylated, enriched with [U-14C]adenosine, and exposed to purine-nucleoside specific enzymes. Reverse phase HPLC and radioactivity measurement demonstrated that for adenosine about 82% of total stable 3H label was in ribose and thus about 18% in adenine. For guanosine about 89% was in ribose and 11% in guanine. This aberrant 3H labelling could be avoided using [1-3H]inositol. Copyright {\textcopyright} 2000 John Wiley & Sons, Ltd.",
author = "S{\o}ren Christensen and H. Klenow and Kay Overgaard-Hansen",
note = "Keywords aberrant 3H; nucleotides; [2-3H]inositol; cell signalling; Ehrlich cell",
year = "2000",
language = "English",
volume = "43",
pages = "91--96",
journal = "Journal of Labelled Compounds and Radiopharmaceuticals",
issn = "0362-4803",
publisher = "JohnWiley & Sons Ltd",
number = "1",

}

RIS

TY - JOUR

T1 - Distribution of 3H within purine nucleotides of Ehrlich mouse ascites tumour cells after intraabdominal injection of myo-[2-3H]inositol

AU - Christensen, Søren

AU - Klenow, H.

AU - Overgaard-Hansen, Kay

N1 - Keywords aberrant 3H; nucleotides; [2-3H]inositol; cell signalling; Ehrlich cell

PY - 2000

Y1 - 2000

N2 - In Ehrlich mouse ascites tumour cells, exposed intra-abdominally to [2-3H]inositol, ATP and GTP presented enough aberrant 3H-label to cause potential interference in the chromatographic analysis of inositol phosphates involved in signal transduction. After acid extraction and charcoal adsorption/desorption the nucleotides were dephosphorylated, enriched with [U-14C]adenosine, and exposed to purine-nucleoside specific enzymes. Reverse phase HPLC and radioactivity measurement demonstrated that for adenosine about 82% of total stable 3H label was in ribose and thus about 18% in adenine. For guanosine about 89% was in ribose and 11% in guanine. This aberrant 3H labelling could be avoided using [1-3H]inositol. Copyright © 2000 John Wiley & Sons, Ltd.

AB - In Ehrlich mouse ascites tumour cells, exposed intra-abdominally to [2-3H]inositol, ATP and GTP presented enough aberrant 3H-label to cause potential interference in the chromatographic analysis of inositol phosphates involved in signal transduction. After acid extraction and charcoal adsorption/desorption the nucleotides were dephosphorylated, enriched with [U-14C]adenosine, and exposed to purine-nucleoside specific enzymes. Reverse phase HPLC and radioactivity measurement demonstrated that for adenosine about 82% of total stable 3H label was in ribose and thus about 18% in adenine. For guanosine about 89% was in ribose and 11% in guanine. This aberrant 3H labelling could be avoided using [1-3H]inositol. Copyright © 2000 John Wiley & Sons, Ltd.

M3 - Journal article

VL - 43

SP - 91

EP - 96

JO - Journal of Labelled Compounds and Radiopharmaceuticals

JF - Journal of Labelled Compounds and Radiopharmaceuticals

SN - 0362-4803

IS - 1

ER -

ID: 79904