Endothelial cell heterogeneity and microglia regulons revealed by a pig cell landscape at single-cell level

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Endothelial cell heterogeneity and microglia regulons revealed by a pig cell landscape at single-cell level. / Wang, Fei; Ding, Peiwen; Liang, Xue; Ding, Xiangning; Brandt, Camilla Blunk; Sjöstedt, Evelina; Zhu, Jiacheng; Bolund, Saga; Zhang, Lijing; de Rooij, Laura P. M. H.; Luo, Lihua; Wei, Yanan; Zhao, Wandong; Lv, Zhiyuan; Haskó, János; Li, Runchu; Qin, Qiuyu; Jia, Yi; Wu, Wendi; Yuan, Yuting; Pu, Mingyi; Wang, Haoyu; Wu, Aiping; Xie, Lin; Liu, Ping; Chen, Fang; Herold, Jacqueline; Kalucka, Joanna; Karlsson, Max; Zhang, Xiuqing; Helmig, Rikke Bek; Fagerberg, Linn; Lindskog, Cecilia; Pontén, Fredrik; Uhlen, Mathias; Bolund, Lars; Jessen, Niels; Jiang, Hui; Xu, Xun; Yang, Huanming; Carmeliet, Peter; Mulder, Jan; Chen, Dongsheng; Lin, Lin; Luo, Yonglun.

I: Nature Communications, Bind 13, 3620, 2022.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Wang, F, Ding, P, Liang, X, Ding, X, Brandt, CB, Sjöstedt, E, Zhu, J, Bolund, S, Zhang, L, de Rooij, LPMH, Luo, L, Wei, Y, Zhao, W, Lv, Z, Haskó, J, Li, R, Qin, Q, Jia, Y, Wu, W, Yuan, Y, Pu, M, Wang, H, Wu, A, Xie, L, Liu, P, Chen, F, Herold, J, Kalucka, J, Karlsson, M, Zhang, X, Helmig, RB, Fagerberg, L, Lindskog, C, Pontén, F, Uhlen, M, Bolund, L, Jessen, N, Jiang, H, Xu, X, Yang, H, Carmeliet, P, Mulder, J, Chen, D, Lin, L & Luo, Y 2022, 'Endothelial cell heterogeneity and microglia regulons revealed by a pig cell landscape at single-cell level', Nature Communications, bind 13, 3620. https://doi.org/10.1038/s41467-022-31388-z

APA

Wang, F., Ding, P., Liang, X., Ding, X., Brandt, C. B., Sjöstedt, E., Zhu, J., Bolund, S., Zhang, L., de Rooij, L. P. M. H., Luo, L., Wei, Y., Zhao, W., Lv, Z., Haskó, J., Li, R., Qin, Q., Jia, Y., Wu, W., ... Luo, Y. (2022). Endothelial cell heterogeneity and microglia regulons revealed by a pig cell landscape at single-cell level. Nature Communications, 13, [3620]. https://doi.org/10.1038/s41467-022-31388-z

Vancouver

Wang F, Ding P, Liang X, Ding X, Brandt CB, Sjöstedt E o.a. Endothelial cell heterogeneity and microglia regulons revealed by a pig cell landscape at single-cell level. Nature Communications. 2022;13. 3620. https://doi.org/10.1038/s41467-022-31388-z

Author

Wang, Fei ; Ding, Peiwen ; Liang, Xue ; Ding, Xiangning ; Brandt, Camilla Blunk ; Sjöstedt, Evelina ; Zhu, Jiacheng ; Bolund, Saga ; Zhang, Lijing ; de Rooij, Laura P. M. H. ; Luo, Lihua ; Wei, Yanan ; Zhao, Wandong ; Lv, Zhiyuan ; Haskó, János ; Li, Runchu ; Qin, Qiuyu ; Jia, Yi ; Wu, Wendi ; Yuan, Yuting ; Pu, Mingyi ; Wang, Haoyu ; Wu, Aiping ; Xie, Lin ; Liu, Ping ; Chen, Fang ; Herold, Jacqueline ; Kalucka, Joanna ; Karlsson, Max ; Zhang, Xiuqing ; Helmig, Rikke Bek ; Fagerberg, Linn ; Lindskog, Cecilia ; Pontén, Fredrik ; Uhlen, Mathias ; Bolund, Lars ; Jessen, Niels ; Jiang, Hui ; Xu, Xun ; Yang, Huanming ; Carmeliet, Peter ; Mulder, Jan ; Chen, Dongsheng ; Lin, Lin ; Luo, Yonglun. / Endothelial cell heterogeneity and microglia regulons revealed by a pig cell landscape at single-cell level. I: Nature Communications. 2022 ; Bind 13.

Bibtex

@article{c016f80b4f6b42768a09b8cbaa203af0,
title = "Endothelial cell heterogeneity and microglia regulons revealed by a pig cell landscape at single-cell level",
abstract = "Pigs are valuable large animal models for biomedical and genetic research, but insights into the tissue- and cell-type-specific transcriptome and heterogeneity remain limited. By leveraging single-cell RNA sequencing, we generate a multiple-organ single-cell transcriptomic map containing over 200,000 pig cells from 20 tissues/organs. We comprehensively characterize the heterogeneity of cells in tissues and identify 234 cell clusters, representing 58 major cell types. In-depth integrative analysis of endothelial cells reveals a high degree of heterogeneity. We identify several functionally distinct endothelial cell phenotypes, including an endothelial to mesenchymal transition subtype in adipose tissues. Intercellular communication analysis predicts tissue- and cell type-specific crosstalk between endothelial cells and other cell types through the VEGF, PDGF, TGF-beta, and BMP pathways. Regulon analysis of single-cell transcriptome of microglia in pig and 12 other species further identifies MEF2C as an evolutionally conserved regulon in the microglia. Our work describes the landscape of single-cell transcriptomes within diverse pig organs and identifies the heterogeneity of endothelial cells and evolutionally conserved regulon in microglia.",
keywords = "TO-MESENCHYMAL TRANSITION, GENE-EXPRESSION, MODEL, ATLAS, ANGIOGENESIS, DIFFERENTIATION, CONTRIBUTES, RESOLUTION, PROVIDE, HEALTH",
author = "Fei Wang and Peiwen Ding and Xue Liang and Xiangning Ding and Brandt, {Camilla Blunk} and Evelina Sj{\"o}stedt and Jiacheng Zhu and Saga Bolund and Lijing Zhang and {de Rooij}, {Laura P. M. H.} and Lihua Luo and Yanan Wei and Wandong Zhao and Zhiyuan Lv and J{\'a}nos Hask{\'o} and Runchu Li and Qiuyu Qin and Yi Jia and Wendi Wu and Yuting Yuan and Mingyi Pu and Haoyu Wang and Aiping Wu and Lin Xie and Ping Liu and Fang Chen and Jacqueline Herold and Joanna Kalucka and Max Karlsson and Xiuqing Zhang and Helmig, {Rikke Bek} and Linn Fagerberg and Cecilia Lindskog and Fredrik Pont{\'e}n and Mathias Uhlen and Lars Bolund and Niels Jessen and Hui Jiang and Xun Xu and Huanming Yang and Peter Carmeliet and Jan Mulder and Dongsheng Chen and Lin Lin and Yonglun Luo",
note = "Correction: DOI 10.1038/s41467-022-34498-w",
year = "2022",
doi = "10.1038/s41467-022-31388-z",
language = "English",
volume = "13",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Endothelial cell heterogeneity and microglia regulons revealed by a pig cell landscape at single-cell level

AU - Wang, Fei

AU - Ding, Peiwen

AU - Liang, Xue

AU - Ding, Xiangning

AU - Brandt, Camilla Blunk

AU - Sjöstedt, Evelina

AU - Zhu, Jiacheng

AU - Bolund, Saga

AU - Zhang, Lijing

AU - de Rooij, Laura P. M. H.

AU - Luo, Lihua

AU - Wei, Yanan

AU - Zhao, Wandong

AU - Lv, Zhiyuan

AU - Haskó, János

AU - Li, Runchu

AU - Qin, Qiuyu

AU - Jia, Yi

AU - Wu, Wendi

AU - Yuan, Yuting

AU - Pu, Mingyi

AU - Wang, Haoyu

AU - Wu, Aiping

AU - Xie, Lin

AU - Liu, Ping

AU - Chen, Fang

AU - Herold, Jacqueline

AU - Kalucka, Joanna

AU - Karlsson, Max

AU - Zhang, Xiuqing

AU - Helmig, Rikke Bek

AU - Fagerberg, Linn

AU - Lindskog, Cecilia

AU - Pontén, Fredrik

AU - Uhlen, Mathias

AU - Bolund, Lars

AU - Jessen, Niels

AU - Jiang, Hui

AU - Xu, Xun

AU - Yang, Huanming

AU - Carmeliet, Peter

AU - Mulder, Jan

AU - Chen, Dongsheng

AU - Lin, Lin

AU - Luo, Yonglun

N1 - Correction: DOI 10.1038/s41467-022-34498-w

PY - 2022

Y1 - 2022

N2 - Pigs are valuable large animal models for biomedical and genetic research, but insights into the tissue- and cell-type-specific transcriptome and heterogeneity remain limited. By leveraging single-cell RNA sequencing, we generate a multiple-organ single-cell transcriptomic map containing over 200,000 pig cells from 20 tissues/organs. We comprehensively characterize the heterogeneity of cells in tissues and identify 234 cell clusters, representing 58 major cell types. In-depth integrative analysis of endothelial cells reveals a high degree of heterogeneity. We identify several functionally distinct endothelial cell phenotypes, including an endothelial to mesenchymal transition subtype in adipose tissues. Intercellular communication analysis predicts tissue- and cell type-specific crosstalk between endothelial cells and other cell types through the VEGF, PDGF, TGF-beta, and BMP pathways. Regulon analysis of single-cell transcriptome of microglia in pig and 12 other species further identifies MEF2C as an evolutionally conserved regulon in the microglia. Our work describes the landscape of single-cell transcriptomes within diverse pig organs and identifies the heterogeneity of endothelial cells and evolutionally conserved regulon in microglia.

AB - Pigs are valuable large animal models for biomedical and genetic research, but insights into the tissue- and cell-type-specific transcriptome and heterogeneity remain limited. By leveraging single-cell RNA sequencing, we generate a multiple-organ single-cell transcriptomic map containing over 200,000 pig cells from 20 tissues/organs. We comprehensively characterize the heterogeneity of cells in tissues and identify 234 cell clusters, representing 58 major cell types. In-depth integrative analysis of endothelial cells reveals a high degree of heterogeneity. We identify several functionally distinct endothelial cell phenotypes, including an endothelial to mesenchymal transition subtype in adipose tissues. Intercellular communication analysis predicts tissue- and cell type-specific crosstalk between endothelial cells and other cell types through the VEGF, PDGF, TGF-beta, and BMP pathways. Regulon analysis of single-cell transcriptome of microglia in pig and 12 other species further identifies MEF2C as an evolutionally conserved regulon in the microglia. Our work describes the landscape of single-cell transcriptomes within diverse pig organs and identifies the heterogeneity of endothelial cells and evolutionally conserved regulon in microglia.

KW - TO-MESENCHYMAL TRANSITION

KW - GENE-EXPRESSION

KW - MODEL

KW - ATLAS

KW - ANGIOGENESIS

KW - DIFFERENTIATION

KW - CONTRIBUTES

KW - RESOLUTION

KW - PROVIDE

KW - HEALTH

U2 - 10.1038/s41467-022-31388-z

DO - 10.1038/s41467-022-31388-z

M3 - Journal article

C2 - 35750885

VL - 13

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 3620

ER -

ID: 312711885