TY - BOOK

T1 - Immune Repertoire Characteristics and Dynamics in Cancer

AU - Liu, Xiao

PY - 2017

Y1 - 2017

N2 - The diversity of T and B cells in terms of their receptors is huge in the invertebrate’simmune system, to provide broad protection against the vast diversity of pathogens.Immune repertoire is defined as the sum of total subtypes that makes the organism’simmune system, either T cell receptor or immunoglobulin. Before the emergence ofhigh-throughput sequencing, the study on immune repertoire is limited bymethodology, as it is impossible to capture the whole picture by the low-throughputtools. The massive paralleled sequencing suits perfectly the study on immunerepertoire. In this thesis, I describe the experimental and analytical methodologies wedeveloped for immune repertoire analysis. We have devoted extensive efforts on theoptimization and evaluation of these pipelines. We then use the tools to investigate thecharacteristics and dynamic of immune repertoire on both blood and solid cancer.Specifically, we provide analysis on leukemic IGH sequences and their evolution inB-cell acute lymphoblastic leukemia, and monitor the dynamics of IGH repertoireduring chemotherapy. Further, the TCR repertoire of infiltrated T lymphocytes inbreast tumor and adjacent tissues was analyzed. We describe numerous interestingfindings that might provide hints on immunotherapy of breast cancer.

AB - The diversity of T and B cells in terms of their receptors is huge in the invertebrate’simmune system, to provide broad protection against the vast diversity of pathogens.Immune repertoire is defined as the sum of total subtypes that makes the organism’simmune system, either T cell receptor or immunoglobulin. Before the emergence ofhigh-throughput sequencing, the study on immune repertoire is limited bymethodology, as it is impossible to capture the whole picture by the low-throughputtools. The massive paralleled sequencing suits perfectly the study on immunerepertoire. In this thesis, I describe the experimental and analytical methodologies wedeveloped for immune repertoire analysis. We have devoted extensive efforts on theoptimization and evaluation of these pipelines. We then use the tools to investigate thecharacteristics and dynamic of immune repertoire on both blood and solid cancer.Specifically, we provide analysis on leukemic IGH sequences and their evolution inB-cell acute lymphoblastic leukemia, and monitor the dynamics of IGH repertoireduring chemotherapy. Further, the TCR repertoire of infiltrated T lymphocytes inbreast tumor and adjacent tissues was analyzed. We describe numerous interestingfindings that might provide hints on immunotherapy of breast cancer.

UR - https://soeg.kb.dk/permalink/45KBDK_KGL/1ed7rpq/alma99122565468705763

M3 - Ph.D. thesis

BT - Immune Repertoire Characteristics and Dynamics in Cancer

PB - Department of Biology, Faculty of Science, University of Copenhagen

ER -