TY - JOUR

T1 - Interleukin 2 and 15 activate Stat3alpha in human T lymphocytes

AU - Nielsen, M

AU - Nordahl, M

AU - Svejgaard, A

AU - Odum, Niels

N1 - Keywords: Acute-Phase Proteins; Cell Nucleus; Cells, Cultured; DNA; DNA-Binding Proteins; Humans; Interleukin-15; Interleukin-2; Phosphorylation; Protein-Tyrosine Kinases; STAT3 Transcription Factor; T-Lymphocytes; Trans-Activators

PY - 1998

Y1 - 1998

N2 - Signal transducer and activator of transcription 3 (Stat3) has recently been shown to exist in two alternatively spliced isoforms, a short form, Stat3beta, and a longer form, Stat3alpha, displaying differences in transcriptional activity. It is unknown which Stat3 isoform(s) is activated in response to interleukin (IL)-2 and IL-15. Here, cytokine-induced activation of Stat3 in previously activated CD4(+) human T cells was examined using Stat3 antibodies directed against different regions of Stat3. As determined by tyrosine phosphorylation, nuclear translocation and binding to an hSIE-oligonucleotide probe, IL-2 and IL-15 activated the slowly migrating isoform, Stat3alpha. In contrast, minimal or no activation of Stat3beta was observed, suggesting that IL-2 and IL-15 predominantly activate Stat3alpha in human CD4(+) T cells. In this way, diversity in the expression and activation of Stat3 proteins may provide additional means of regulating cytokine-induced T cell responses.

AB - Signal transducer and activator of transcription 3 (Stat3) has recently been shown to exist in two alternatively spliced isoforms, a short form, Stat3beta, and a longer form, Stat3alpha, displaying differences in transcriptional activity. It is unknown which Stat3 isoform(s) is activated in response to interleukin (IL)-2 and IL-15. Here, cytokine-induced activation of Stat3 in previously activated CD4(+) human T cells was examined using Stat3 antibodies directed against different regions of Stat3. As determined by tyrosine phosphorylation, nuclear translocation and binding to an hSIE-oligonucleotide probe, IL-2 and IL-15 activated the slowly migrating isoform, Stat3alpha. In contrast, minimal or no activation of Stat3beta was observed, suggesting that IL-2 and IL-15 predominantly activate Stat3alpha in human CD4(+) T cells. In this way, diversity in the expression and activation of Stat3 proteins may provide additional means of regulating cytokine-induced T cell responses.

U2 - 10.1006/cyto.1998.0356

DO - 10.1006/cyto.1998.0356

M3 - Journal article

C2 - 9811525

VL - 10

SP - 735

EP - 738

JO - Cytokine

JF - Cytokine

SN - 1043-4666

IS - 10

ER -