Molecular characterization of SAT 2 foot-and-mouth disease virus from post-outbreak slaughtered animals: implications for disease control in Uganda
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Molecular characterization of SAT 2 foot-and-mouth disease virus from post-outbreak slaughtered animals: implications for disease control in Uganda. / Balinda, Sheila; Belsham, Graham; Masembe, C; Sangula, Abraham K.; Siegismund, H R; Muwanika, V B.
I: Epidemiology and Infection, Bind 138, Nr. 8, 01.08.2010, s. 1204-10.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Molecular characterization of SAT 2 foot-and-mouth disease virus from post-outbreak slaughtered animals: implications for disease control in Uganda
AU - Balinda, Sheila
AU - Belsham, Graham
AU - Masembe, C
AU - Sangula, Abraham K.
AU - Siegismund, H R
AU - Muwanika, V B
PY - 2010/8/1
Y1 - 2010/8/1
N2 - In Uganda, limiting the extent of foot-and-mouth disease (FMD) spread during outbreaks involves short-term measures such as ring vaccination and restrictions of the movement of livestock and their products to and from the affected areas. In this study, the presence of FMD virus RNA was investigated in cattle samples 3 months after FMD quarantine measures had been lifted following an outbreak in 2004. Oropharyngeal tissue samples were obtained from 12 cattle slaughtered in a small town abattoir in Kiboga. FMD virus RNA was detected by diagnostic RT-PCR in nine of the 12 tissue samples. Part of the coding region for the capsid protein VP1 was amplified and sequenced. All samples were identified as belonging to the SAT 2 serotype. The implications for FMD control of both virus introduction into Uganda and the presence of carrier animals following outbreaks are discussed.
AB - In Uganda, limiting the extent of foot-and-mouth disease (FMD) spread during outbreaks involves short-term measures such as ring vaccination and restrictions of the movement of livestock and their products to and from the affected areas. In this study, the presence of FMD virus RNA was investigated in cattle samples 3 months after FMD quarantine measures had been lifted following an outbreak in 2004. Oropharyngeal tissue samples were obtained from 12 cattle slaughtered in a small town abattoir in Kiboga. FMD virus RNA was detected by diagnostic RT-PCR in nine of the 12 tissue samples. Part of the coding region for the capsid protein VP1 was amplified and sequenced. All samples were identified as belonging to the SAT 2 serotype. The implications for FMD control of both virus introduction into Uganda and the presence of carrier animals following outbreaks are discussed.
KW - Animals
KW - Base Composition
KW - Base Sequence
KW - Capsid Proteins
KW - Cattle
KW - Disease Outbreaks
KW - Foot-and-Mouth Disease
KW - Foot-and-Mouth Disease Virus
KW - Molecular Sequence Data
KW - Phylogeny
KW - Quarantine
KW - RNA, Viral
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Sequence Alignment
KW - Uganda
U2 - 10.1017/S0950268809991427
DO - 10.1017/S0950268809991427
M3 - Journal article
C2 - 20003615
VL - 138
SP - 1204
EP - 1210
JO - Epidemiology and Infection
JF - Epidemiology and Infection
SN - 0950-2688
IS - 8
ER -
ID: 33289840