Protocol for Purification of Human ZGRF1 and Its Regulatory Function on RAD51-Mediated D-Loop Formation

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Standard

Protocol for Purification of Human ZGRF1 and Its Regulatory Function on RAD51-Mediated D-Loop Formation. / Lisby, Michael; Xue, Xiaoyu.

I: STAR Protocols, Bind 1, Nr. 2, 2020.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Lisby, M & Xue, X 2020, 'Protocol for Purification of Human ZGRF1 and Its Regulatory Function on RAD51-Mediated D-Loop Formation', STAR Protocols, bind 1, nr. 2. https://doi.org/10.1016/j.xpro.2020.100099

APA

Lisby, M., & Xue, X. (2020). Protocol for Purification of Human ZGRF1 and Its Regulatory Function on RAD51-Mediated D-Loop Formation. STAR Protocols, 1(2). https://doi.org/10.1016/j.xpro.2020.100099

Vancouver

Lisby M, Xue X. Protocol for Purification of Human ZGRF1 and Its Regulatory Function on RAD51-Mediated D-Loop Formation. STAR Protocols. 2020;1(2). https://doi.org/10.1016/j.xpro.2020.100099

Author

Lisby, Michael ; Xue, Xiaoyu. / Protocol for Purification of Human ZGRF1 and Its Regulatory Function on RAD51-Mediated D-Loop Formation. I: STAR Protocols. 2020 ; Bind 1, Nr. 2.

Bibtex

@article{2505420c1ed4467c8417a2c7f0743e4d,
title = "Protocol for Purification of Human ZGRF1 and Its Regulatory Function on RAD51-Mediated D-Loop Formation",
abstract = "Human ZGRF1 is one of the many helicases that play important roles in the repair of damaged chromosomes by homologous recombination. However, the lack of biochemical characterization of the ZGRF1 protein has hindered the understanding of its function. We have developed a facile protocol to express human ZGRF1 in insect cells and for its purification. We have also tuned biochemical assays by lowering the protein and DNA concentrations to analyze its functions in RAD51-mediated D-loop formation and dissociation. For complete details on the use and execution of this protocol, please refer to Brannvoll et al. (2020).",
author = "Michael Lisby and Xiaoyu Xue",
year = "2020",
doi = "10.1016/j.xpro.2020.100099",
language = "English",
volume = "1",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",

}

RIS

TY - JOUR

T1 - Protocol for Purification of Human ZGRF1 and Its Regulatory Function on RAD51-Mediated D-Loop Formation

AU - Lisby, Michael

AU - Xue, Xiaoyu

PY - 2020

Y1 - 2020

N2 - Human ZGRF1 is one of the many helicases that play important roles in the repair of damaged chromosomes by homologous recombination. However, the lack of biochemical characterization of the ZGRF1 protein has hindered the understanding of its function. We have developed a facile protocol to express human ZGRF1 in insect cells and for its purification. We have also tuned biochemical assays by lowering the protein and DNA concentrations to analyze its functions in RAD51-mediated D-loop formation and dissociation. For complete details on the use and execution of this protocol, please refer to Brannvoll et al. (2020).

AB - Human ZGRF1 is one of the many helicases that play important roles in the repair of damaged chromosomes by homologous recombination. However, the lack of biochemical characterization of the ZGRF1 protein has hindered the understanding of its function. We have developed a facile protocol to express human ZGRF1 in insect cells and for its purification. We have also tuned biochemical assays by lowering the protein and DNA concentrations to analyze its functions in RAD51-mediated D-loop formation and dissociation. For complete details on the use and execution of this protocol, please refer to Brannvoll et al. (2020).

U2 - 10.1016/j.xpro.2020.100099

DO - 10.1016/j.xpro.2020.100099

M3 - Journal article

C2 - 32984852

VL - 1

JO - STAR Protocols

JF - STAR Protocols

SN - 2666-1667

IS - 2

ER -

ID: 258411151