Repression of a mating type cassette in the fission yeast by four DNA elements
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Repression of a mating type cassette in the fission yeast by four DNA elements. / Ekwall, K; Nielsen, O; Ruusala, T; Nielsen, Olaf.
I: Yeast, Bind 7, Nr. 7, 01.10.1991, s. 745-55.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Repression of a mating type cassette in the fission yeast by four DNA elements
AU - Ekwall, K
AU - Nielsen, O
AU - Ruusala, T
AU - Nielsen, Olaf
PY - 1991/10/1
Y1 - 1991/10/1
N2 - The fission yeast, Schizosaccharomyces pombe, expresses one of two alternative mating types. They are specified by one of two determinants (M or P) present at the mat1 locus. In addition, silent copies of M and P are present on the same chromosome. In the present work we demonstrate that the difference between the active and the silent stage of the P determinant is controlled by four repressive elements that are located at the silent locus. There are two elements to the left and two to the right of the mating type cassette. Both elements to the left and either one of the two elements to the right are required for an effective blockage of transcription. When they are combined, the four elements define a highly efficient silencer functionally similar to the HMRE and HMLE and HMLI silencers in Saccharomyces cerevisiae. In addition, the DNA surrounding the silent P locus confers symmetric partitioning in mitosis to Schizosaccharomyces pombe ars plasmids.
AB - The fission yeast, Schizosaccharomyces pombe, expresses one of two alternative mating types. They are specified by one of two determinants (M or P) present at the mat1 locus. In addition, silent copies of M and P are present on the same chromosome. In the present work we demonstrate that the difference between the active and the silent stage of the P determinant is controlled by four repressive elements that are located at the silent locus. There are two elements to the left and two to the right of the mating type cassette. Both elements to the left and either one of the two elements to the right are required for an effective blockage of transcription. When they are combined, the four elements define a highly efficient silencer functionally similar to the HMRE and HMLE and HMLI silencers in Saccharomyces cerevisiae. In addition, the DNA surrounding the silent P locus confers symmetric partitioning in mitosis to Schizosaccharomyces pombe ars plasmids.
KW - DNA, Fungal
KW - Gene Expression Regulation, Fungal
KW - Genes, Fungal
KW - Genes, Mating Type, Fungal
KW - Mitosis
KW - Plasmids
KW - Regulatory Sequences, Nucleic Acid
KW - Schizosaccharomyces
U2 - 10.1002/yea.320070709
DO - 10.1002/yea.320070709
M3 - Journal article
C2 - 1776364
VL - 7
SP - 745
EP - 755
JO - Yeast
JF - Yeast
SN - 0749-503X
IS - 7
ER -
ID: 33577574