Ribotyping on small-sized spirochetes isolated from subgingival plaque.

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Ribotyping on small-sized spirochetes isolated from subgingival plaque. / Fiehn, N E; Bangsborg, J M; Colding, H.

I: Oral Microbiology and Immunology, Bind 10, Nr. 1, 1995, s. 13-8.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Fiehn, NE, Bangsborg, JM & Colding, H 1995, 'Ribotyping on small-sized spirochetes isolated from subgingival plaque.', Oral Microbiology and Immunology, bind 10, nr. 1, s. 13-8.

APA

Fiehn, N. E., Bangsborg, J. M., & Colding, H. (1995). Ribotyping on small-sized spirochetes isolated from subgingival plaque. Oral Microbiology and Immunology, 10(1), 13-8.

Vancouver

Fiehn NE, Bangsborg JM, Colding H. Ribotyping on small-sized spirochetes isolated from subgingival plaque. Oral Microbiology and Immunology. 1995;10(1):13-8.

Author

Fiehn, N E ; Bangsborg, J M ; Colding, H. / Ribotyping on small-sized spirochetes isolated from subgingival plaque. I: Oral Microbiology and Immunology. 1995 ; Bind 10, Nr. 1. s. 13-8.

Bibtex

@article{71087bb0b55211ddb04f000ea68e967b,
title = "Ribotyping on small-sized spirochetes isolated from subgingival plaque.",
abstract = "In the present study DNA restriction patterns and corresponding ribotypes of 17 subgingival small-sized spirochetes (1:2:1 and 2:4:2 isolates), 2 Treponema socranskii strains and two Treponema denticola strains were examined. Purified chromosomal DNA was digested by BamHI, HindIII, PstI and ClaI. The DNA fragments were separated in a horizontal slab of 0.7% agarose containing ethidium bromide and transferred by nylon membranes. Hybridization was carried out with digoxigenin-labelled copy DNA of 16S and 23S ribosomal RNA from Escherichia coli. Depending on the restriction endonuclease used, up to 4 distinct bands were observed for the 2:4:2 isolates and the T. denticola strains. For each of the endonucleases used, identical band patterns were always observed for this group of isolates, and these patterns differed persistently from the T. denticola strains. For the 1:2:1 strains, up to 11 distinct bands were observed after digestion with HindIII, whereas a maximum of 6 bands were observed when PstI or ClaI was used. By using ClaI, the examined 1:2:1 isolates were separated into 8 groups, whereas PstI and HindIII separated these isolates into 5 groups. The ribotyping showed that the tested 1:2:1 spirochetes were more heterogeneous than the 2:4:2 spirochetes examined.",
author = "Fiehn, {N E} and Bangsborg, {J M} and H Colding",
note = "Keywords: Bacterial Typing Techniques; DNA Probes; DNA Restriction Enzymes; DNA, Bacterial; Dental Plaque; Humans; Periodontal Pocket; RNA, Bacterial; RNA, Ribosomal, 16S; RNA, Ribosomal, 23S; Treponema",
year = "1995",
language = "English",
volume = "10",
pages = "13--8",
journal = "Oral Microbiology and Immunology",
issn = "0902-0055",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS

TY - JOUR

T1 - Ribotyping on small-sized spirochetes isolated from subgingival plaque.

AU - Fiehn, N E

AU - Bangsborg, J M

AU - Colding, H

N1 - Keywords: Bacterial Typing Techniques; DNA Probes; DNA Restriction Enzymes; DNA, Bacterial; Dental Plaque; Humans; Periodontal Pocket; RNA, Bacterial; RNA, Ribosomal, 16S; RNA, Ribosomal, 23S; Treponema

PY - 1995

Y1 - 1995

N2 - In the present study DNA restriction patterns and corresponding ribotypes of 17 subgingival small-sized spirochetes (1:2:1 and 2:4:2 isolates), 2 Treponema socranskii strains and two Treponema denticola strains were examined. Purified chromosomal DNA was digested by BamHI, HindIII, PstI and ClaI. The DNA fragments were separated in a horizontal slab of 0.7% agarose containing ethidium bromide and transferred by nylon membranes. Hybridization was carried out with digoxigenin-labelled copy DNA of 16S and 23S ribosomal RNA from Escherichia coli. Depending on the restriction endonuclease used, up to 4 distinct bands were observed for the 2:4:2 isolates and the T. denticola strains. For each of the endonucleases used, identical band patterns were always observed for this group of isolates, and these patterns differed persistently from the T. denticola strains. For the 1:2:1 strains, up to 11 distinct bands were observed after digestion with HindIII, whereas a maximum of 6 bands were observed when PstI or ClaI was used. By using ClaI, the examined 1:2:1 isolates were separated into 8 groups, whereas PstI and HindIII separated these isolates into 5 groups. The ribotyping showed that the tested 1:2:1 spirochetes were more heterogeneous than the 2:4:2 spirochetes examined.

AB - In the present study DNA restriction patterns and corresponding ribotypes of 17 subgingival small-sized spirochetes (1:2:1 and 2:4:2 isolates), 2 Treponema socranskii strains and two Treponema denticola strains were examined. Purified chromosomal DNA was digested by BamHI, HindIII, PstI and ClaI. The DNA fragments were separated in a horizontal slab of 0.7% agarose containing ethidium bromide and transferred by nylon membranes. Hybridization was carried out with digoxigenin-labelled copy DNA of 16S and 23S ribosomal RNA from Escherichia coli. Depending on the restriction endonuclease used, up to 4 distinct bands were observed for the 2:4:2 isolates and the T. denticola strains. For each of the endonucleases used, identical band patterns were always observed for this group of isolates, and these patterns differed persistently from the T. denticola strains. For the 1:2:1 strains, up to 11 distinct bands were observed after digestion with HindIII, whereas a maximum of 6 bands were observed when PstI or ClaI was used. By using ClaI, the examined 1:2:1 isolates were separated into 8 groups, whereas PstI and HindIII separated these isolates into 5 groups. The ribotyping showed that the tested 1:2:1 spirochetes were more heterogeneous than the 2:4:2 spirochetes examined.

M3 - Journal article

C2 - 7543994

VL - 10

SP - 13

EP - 18

JO - Oral Microbiology and Immunology

JF - Oral Microbiology and Immunology

SN - 0902-0055

IS - 1

ER -

ID: 8670073