RNA-binding IMPs promote cell adhesion and invadopodia formation.
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RNA-binding IMPs promote cell adhesion and invadopodia formation. / Vikesaa, Jonas; Hansen, Thomas V O; Jønson, Lars; Borup, Rehannah; Wewer, Ulla M; Christiansen, Jan; Nielsen, Finn C.
I: EMBO Journal, Bind 25, Nr. 7, 2006, s. 1456-68.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - RNA-binding IMPs promote cell adhesion and invadopodia formation.
AU - Vikesaa, Jonas
AU - Hansen, Thomas V O
AU - Jønson, Lars
AU - Borup, Rehannah
AU - Wewer, Ulla M
AU - Christiansen, Jan
AU - Nielsen, Finn C
N1 - Keywords: 3' Untranslated Regions; Active Transport, Cell Nucleus; Antigens, CD44; Binding Sites; Cell Adhesion; Cell Nucleus; Cell Shape; Cell Surface Extensions; Extracellular Matrix; Hela Cells; Humans; Neoplasm Proteins; RNA Interference; RNA Processing, Post-Transcriptional; RNA Stability; RNA, Messenger; RNA-Binding Proteins
PY - 2006
Y1 - 2006
N2 - Oncofetal RNA-binding IMPs have been implicated in mRNA localization, nuclear export, turnover and translational control. To depict the cellular actions of IMPs, we performed a loss-of-function analysis, which showed that IMPs are necessary for proper cell adhesion, cytoplasmic spreading and invadopodia formation. Loss of IMPs was associated with a coordinate downregulation of mRNAs encoding extracellular matrix and adhesion proteins. The transcripts were present in IMP RNP granules, implying that IMPs were directly involved in the post-transcriptional control of the transcripts. In particular, we show that a 5.0 kb CD44 mRNA contained multiple IMP-binding sites in its 3'UTR, and following IMP depletion this species became unstable. Direct knockdown of the CD44 transcript mimicked the effect of IMPs on invadopodia, and we infer that CD44 mRNA stabilization may be involved in IMP-mediated invadopodia formation. Taken together, our results indicate that RNA-binding proteins exert profound effects on cellular adhesion and invasion during development and cancer formation.
AB - Oncofetal RNA-binding IMPs have been implicated in mRNA localization, nuclear export, turnover and translational control. To depict the cellular actions of IMPs, we performed a loss-of-function analysis, which showed that IMPs are necessary for proper cell adhesion, cytoplasmic spreading and invadopodia formation. Loss of IMPs was associated with a coordinate downregulation of mRNAs encoding extracellular matrix and adhesion proteins. The transcripts were present in IMP RNP granules, implying that IMPs were directly involved in the post-transcriptional control of the transcripts. In particular, we show that a 5.0 kb CD44 mRNA contained multiple IMP-binding sites in its 3'UTR, and following IMP depletion this species became unstable. Direct knockdown of the CD44 transcript mimicked the effect of IMPs on invadopodia, and we infer that CD44 mRNA stabilization may be involved in IMP-mediated invadopodia formation. Taken together, our results indicate that RNA-binding proteins exert profound effects on cellular adhesion and invasion during development and cancer formation.
U2 - 10.1038/sj.emboj.7601039
DO - 10.1038/sj.emboj.7601039
M3 - Journal article
C2 - 16541107
VL - 25
SP - 1456
EP - 1468
JO - E M B O Journal
JF - E M B O Journal
SN - 0261-4189
IS - 7
ER -
ID: 6474220