A microsatellite-based method for genotyping diploid and triploid water frogs of the Rana esculenta hybrid complex

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A microsatellite-based method for genotyping diploid and triploid water frogs of the Rana esculenta hybrid complex. / Christiansen, Ditte Guldager.

In: Molecular Ecology Notes, Vol. 5, No. 1, 2005, p. 190-193.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Christiansen, DG 2005, 'A microsatellite-based method for genotyping diploid and triploid water frogs of the Rana esculenta hybrid complex', Molecular Ecology Notes, vol. 5, no. 1, pp. 190-193. https://doi.org/10.1111/j.1471-8286.2004.00869.x

APA

Christiansen, D. G. (2005). A microsatellite-based method for genotyping diploid and triploid water frogs of the Rana esculenta hybrid complex. Molecular Ecology Notes, 5(1), 190-193. https://doi.org/10.1111/j.1471-8286.2004.00869.x

Vancouver

Christiansen DG. A microsatellite-based method for genotyping diploid and triploid water frogs of the Rana esculenta hybrid complex. Molecular Ecology Notes. 2005;5(1):190-193. https://doi.org/10.1111/j.1471-8286.2004.00869.x

Author

Christiansen, Ditte Guldager. / A microsatellite-based method for genotyping diploid and triploid water frogs of the Rana esculenta hybrid complex. In: Molecular Ecology Notes. 2005 ; Vol. 5, No. 1. pp. 190-193.

Bibtex

@article{4746841074c311dbbee902004c4f4f50,
title = "A microsatellite-based method for genotyping diploid and triploid water frogs of the Rana esculenta hybrid complex",
abstract = "Rana esculenta is a hybrid between Rana lessonae (LL) and Rana ridibunda (RR), and hybrids may be diploid (LR) or triploid (LLR or LRR). Genotypes can be roughly determined from erythrocyte size and morphometry in adult frogs, but accurate genotyping requires more labourious methods. Here I demonstrate that both the L and R genomes have specific microsatellite alleles, and that genotype and ploidy can be accurately inferred from the quantitative ratio of PCR-amplified (polymerase chain reaction-amplified) genome-specific alleles. This method greatly facilitates genotyping in DNA studies of the R. esculenta complex and allows analysis of badly preserved samples and embryos.",
author = "Christiansen, {Ditte Guldager}",
note = "KEYWORDS competitive PCR • genotyping • microsatellites • Rana esculenta",
year = "2005",
doi = "10.1111/j.1471-8286.2004.00869.x",
language = "English",
volume = "5",
pages = "190--193",
journal = "Molecular Ecology",
issn = "0962-1083",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS

TY - JOUR

T1 - A microsatellite-based method for genotyping diploid and triploid water frogs of the Rana esculenta hybrid complex

AU - Christiansen, Ditte Guldager

N1 - KEYWORDS competitive PCR • genotyping • microsatellites • Rana esculenta

PY - 2005

Y1 - 2005

N2 - Rana esculenta is a hybrid between Rana lessonae (LL) and Rana ridibunda (RR), and hybrids may be diploid (LR) or triploid (LLR or LRR). Genotypes can be roughly determined from erythrocyte size and morphometry in adult frogs, but accurate genotyping requires more labourious methods. Here I demonstrate that both the L and R genomes have specific microsatellite alleles, and that genotype and ploidy can be accurately inferred from the quantitative ratio of PCR-amplified (polymerase chain reaction-amplified) genome-specific alleles. This method greatly facilitates genotyping in DNA studies of the R. esculenta complex and allows analysis of badly preserved samples and embryos.

AB - Rana esculenta is a hybrid between Rana lessonae (LL) and Rana ridibunda (RR), and hybrids may be diploid (LR) or triploid (LLR or LRR). Genotypes can be roughly determined from erythrocyte size and morphometry in adult frogs, but accurate genotyping requires more labourious methods. Here I demonstrate that both the L and R genomes have specific microsatellite alleles, and that genotype and ploidy can be accurately inferred from the quantitative ratio of PCR-amplified (polymerase chain reaction-amplified) genome-specific alleles. This method greatly facilitates genotyping in DNA studies of the R. esculenta complex and allows analysis of badly preserved samples and embryos.

U2 - 10.1111/j.1471-8286.2004.00869.x

DO - 10.1111/j.1471-8286.2004.00869.x

M3 - Journal article

VL - 5

SP - 190

EP - 193

JO - Molecular Ecology

JF - Molecular Ecology

SN - 0962-1083

IS - 1

ER -

ID: 89667