A new scoring function for protein-protein docking that identifies native structures with unprecedented accuracy
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A new scoring function for protein-protein docking that identifies native structures with unprecedented accuracy. / Moreira, Irina S.; da Silva Martins, João Miguel; Coimbra, João T.S.; Ramos, Maria J.; Fernandes, Pedro A.
In: Physical Chemistry Chemical Physics, Vol. 17, No. 4, 2015, p. 2378-2387.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - A new scoring function for protein-protein docking that identifies native structures with unprecedented accuracy
AU - Moreira, Irina S.
AU - da Silva Martins, João Miguel
AU - Coimbra, João T.S.
AU - Ramos, Maria J.
AU - Fernandes, Pedro A.
PY - 2015
Y1 - 2015
N2 - Protein-protein (P-P) 3D structures are fundamental to structural biology and drug discovery. However, most of them have never been determined. Many docking algorithms were developed for that purpose, but they have a very limited accuracy in generating native-like structures and identifying the most correct one, in particular when a single answer is asked for. With such a low success rate it is difficult to point out one docked structure as being native-like. Here we present a new, high accuracy, scoring method to identify the 3D structure of P-P complexes among a set of trial poses. It incorporates alanine scanning mutagenesis experimental data that need to be obtained a priori. The scoring scheme works by matching the computational and the experimental alanine scanning mutagenesis results. The size of the trial P-P interface area is also taken into account. We show that the method ranks the trial structures and identifies the native-like structures with unprecedented accuracy (∼94%), providing the correct P-P 3D structures that biochemists and molecular biologists need to pursue their studies. With such a success rate, the bottleneck of protein-protein docking moves from the scoring to searching algorithms. This journal is
AB - Protein-protein (P-P) 3D structures are fundamental to structural biology and drug discovery. However, most of them have never been determined. Many docking algorithms were developed for that purpose, but they have a very limited accuracy in generating native-like structures and identifying the most correct one, in particular when a single answer is asked for. With such a low success rate it is difficult to point out one docked structure as being native-like. Here we present a new, high accuracy, scoring method to identify the 3D structure of P-P complexes among a set of trial poses. It incorporates alanine scanning mutagenesis experimental data that need to be obtained a priori. The scoring scheme works by matching the computational and the experimental alanine scanning mutagenesis results. The size of the trial P-P interface area is also taken into account. We show that the method ranks the trial structures and identifies the native-like structures with unprecedented accuracy (∼94%), providing the correct P-P 3D structures that biochemists and molecular biologists need to pursue their studies. With such a success rate, the bottleneck of protein-protein docking moves from the scoring to searching algorithms. This journal is
U2 - 10.1039/c4cp04688a
DO - 10.1039/c4cp04688a
M3 - Journal article
C2 - 25490550
AN - SCOPUS:84919807951
VL - 17
SP - 2378
EP - 2387
JO - Physical Chemistry Chemical Physics
JF - Physical Chemistry Chemical Physics
SN - 1463-9076
IS - 4
ER -
ID: 153377409